The loss of lectin reactivity from human endometrium is a feature of malignant change

Pathology, Research and Practice
E Sivridis, N Agnantis

Abstract

Changes in the cell surface glycoproteins were investigated in endometrial adenocarcinomas using eight biotin-labelled lectins (Con A, LCA, WGA, e-PHA, l-PHA, SBA, PNA, LTA) which select for the major classes of N-linked and O-linked oligosaccharides. The tissues studied were 14 normal endometria, 20 cases of simple hyperplasia and 30 endometrial adenocarcinomas. Sections were cut at 5 microns from formalin fixed paraffin embedded blocks and were stained with an avidin-peroxidase method. All normal and hyperplastic endometria contained the full range of expected saccharides (positive staining for Con A, LCA, WGA, SBA, PNA, LTA, e-PHA and l-PHA). By contrast, over half of the endometrial adenocarcinomas exhibited a loss of galactosamine (56.7%) and over a third of them failed to reveal galactose (36.7%) and fucose (30.0%) (negative staining for SBA, PNA and LTA, respectively). Interestingly, following neuraminidase treatment, galactose was identified in all adenocarcinomas studied, but the expression of galactosamine and fucose was unaffected. There was a discrete linear staining at the level of the basement membranes in a proportion of endometrial glands treated with e-PHA, l-PHA and LCA; this was continuous in normal and hyper...Continue Reading

References

Jan 1, 1991·Gynecologic and Obstetric Investigation·V BychkovV B Reddy
Mar 1, 1988·Journal of Clinical Pathology·K O Söderström
Apr 1, 1985·The Journal of Pathology·P BirembautJ M Foidart
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Nov 1, 1983·The Journal of Pathology·M J CrumptonA A Davies
Mar 1, 1993·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·E. SivridisH. Fox

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Citations

Feb 15, 2001·Pathology Oncology Research : POR·A C LazarisP S Davaris
Aug 23, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·E SivridisP Anastasiadis
Jun 15, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Fabiana H M de MeloRoger Chammas

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