The ltp gene of temperate Streptococcus thermophilus phage TP-J34 confers superinfection exclusion to Streptococcus thermophilus and Lactococcus lactis

Virology
Xingmin SunHorst Neve

Abstract

The ltp gene, located within the lysogeny module of temperate Streptococcus thermophilus phage TP-J34, has been shown to be expressed in lysogenic strain S. thermophilus J34. It codes for a lipoprotein, as demonstrated by inhibition of cleavage of the signal sequence by globomycin. Exposure of Ltp on the surface of Lactococcus lactis protoplasts bearing a plasmid-encoded copy of ltp has been demonstrated by immunogold labeling and electron microscopy. Expression of ltp in prophage- and plasmid-cured S. thermophilus J34-6f interfered with TP-J34 infection. While plating efficiency was reduced by a factor of about 40 and lysis of strain J34-6f in liquid medium was delayed considerably, phage adsorption was not affected at all. Intracellular accumulation of phage DNA was shown to be inhibited by Ltp. This indicates interference of Ltp with infection at the stage of triggering DNA release and injection into the cell, indicating a role of Ltp in superinfection exclusion. Expression of ltp in L. lactis Bu2-60 showed that the same superinfection exclusion mechanism was strongly effective against phage P008, a member of the lactococcal 936 phage species: no plaque-formation was detectable with even 10(9) phage per ml applied, and lysis...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1991·Intervirology·A W JarvisM Teuber
May 1, 1989·Protein Engineering·G von Heijne
Sep 15, 1986·FEBS Letters·I Riede, M L Eschbach
Oct 1, 1995·Microbiology·G R ArmoaS L Morris
Nov 1, 1995·Journal of Bacteriology·R MerchanteD Karamata
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchultzC P Ponting
May 3, 2002·Molecular Microbiology·Stephen McGrathDouwe van Sinderen
Dec 3, 2003·Virology·Marco VenturaHarald Brüssow
Feb 20, 2004·Virology·Mary D RobertsAndrew M Kropinski
Jan 1, 1991·Applied and Environmental Microbiology·C HillT R Klaenhammer

❮ Previous
Next ❯

Citations

Mar 4, 2014·The Journal of Microbiology·Joseph Bondy-Denomy, Alan R Davidson
Sep 5, 2014·Microbial Cell Factories·Jennifer MahonyGerald F Fitzgerald
Dec 19, 2012·Annual Review of Food Science and Technology·Julie E Samson, Sylvain Moineau
Nov 3, 2014·Annual Review of Virology·Ron L DyPeter C Fineran
Feb 12, 2008·Journal of Bacteriology·Andreas AmmannKnut J Heller
Aug 30, 2008·Applied and Environmental Microbiology·Jennifer MahonyDouwe van Sinderen
Nov 10, 2011·Viruses·Luis P Villarreal
Aug 28, 2007·Current Opinion in Microbiology·Paul A Hoskisson, Margaret C M Smith
Jul 17, 2015·Molecular Microbiology·Ognjen SekulovicLouis-Charles Fortier
Jun 4, 2016·The ISME Journal·Joseph Bondy-DenomyKaren L Maxwell
Sep 28, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Frank Hille, Emmanuelle Charpentier
Nov 29, 2017·Applied and Environmental Microbiology·Brian McDonnellDouwe van Sinderen
Oct 23, 2015·Journal of Virology·Livia DonaireFernando García-Arenal
Jul 15, 2016·Microbiology and Molecular Biology Reviews : MMBR·Stineke van HouteEdze R Westra
Apr 9, 2013·Applied and Environmental Microbiology·Yahya AliHorst Neve
Dec 17, 2014·Applied and Environmental Microbiology·Ognjen Sekulovic, Louis-Charles Fortier
Jun 19, 2016·Applied and Environmental Microbiology·Brian McDonnellDouwe van Sinderen
Mar 30, 2010·Nature Reviews. Microbiology·Simon J LabrieSylvain Moineau
Mar 25, 2019·Applied and Environmental Microbiology·Abraham G MollerTimothy D Read
Apr 11, 2020·Microorganisms·Pilar Domingo-CalapRafael Sanjuán
Apr 9, 2020·Frontiers in Microbiology·Jaime IranzoEugene V Koonin
May 27, 2021·Cell Reports·Amany M Al-AnanyAlexander P Hynes
Jun 22, 2021·Frontiers in Cellular and Infection Microbiology·Eleanor M TownsendEleanor Jameson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.