The LuxS/AI-2 Quorum-Sensing System of Streptococcus pneumoniae Is Required to Cause Disease, and to Regulate Virulence- and Metabolism-Related Genes in a Rat Model of Middle Ear Infection

Frontiers in Cellular and Infection Microbiology
Mukesh Kumar YadavJae-Jun Song

Abstract

Objective:Streptococcus pneumoniae colonizes the nasopharynx of children, and from nasopharynx it could migrate to the middle ear and causes acute otitis media (AOM). During colonization and AOM, the pneumococcus forms biofilms. In vitro biofilm formation requires a functional LuxS/AI-2 quorum-sensing system. We investigated the role of LuxS/AI-2 signaling in pneumococcal middle ear infection, and identified the genes that are regulated by LuxS/AI-2 during pneumococcal biofilm formation. Methods:Streptococcus pneumoniae D39 wild-type and an isogenic D39ΔluxS strain were utilized to evaluate in vitro biofilm formation, and in vivo colonization and epithelial damage using a microtiter plate assay and a rat model of pneumococcal middle ear infection, respectively. Biofilm structures and colonization and epithelial damage were evaluated at the ultrastructural level by scanning electron microscopy and confocal microscopy. Microarrays were used to investigate the global genes that were regulated by LuxS/AI-2 during biofilm formation. Results: The biofilm biomass and density of D39ΔluxS were significantly (p < 0.05) lower than those of D39 wild-type. SEM and confocal microscopy revealed that D39ΔluxS formed thin biofilms in vitro comp...Continue Reading

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Citations

Sep 11, 2019·Critical Reviews in Microbiology·Maria Daniela Silva, Sanna Sillankorva
Feb 26, 2019·Frontiers in Cellular and Infection Microbiology·Osmel Fleitas MartínezOctavio Luiz Franco
Nov 5, 2019·Frontiers in Microbiology·Uriel A Angulo-ZamudioNidia León-Sicairos
Jul 1, 2020·BioMed Research International·Min YangJie Yu
Nov 19, 2019·Frontiers in Microbiology·Paweł Krzyżek
Nov 26, 2020·Microorganisms·Puja YadavBarbara Spellerberg
Oct 23, 2018·European Journal of Medicinal Chemistry·Barbara ParrinoStella Cascioferro
Dec 28, 2019·International Journal of Pediatric Otorhinolaryngology·R B ThorntonK L Brockman
Oct 17, 2020·Molecular Oral Microbiology·Ok-Jin ParkSeung Hyun Han
Dec 18, 2020·Comprehensive Reviews in Food Science and Food Safety·Dan ZhangHarold Corke
Nov 3, 2020·Expert Review of Anti-infective Therapy·Mainul HaqueDilshad Jahan
Feb 18, 2020·Microbial Pathogenesis·Erika Kushikawa SaekiGerson Nakazato
Mar 1, 2021·Archives of Microbiology·Sarika SureshRamya Premanath

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Datasets Mentioned

BETA
GSE109347

Methods Mentioned

BETA
confocal microscopy
PCR

Software Mentioned

Genowiz
Global LOWESS
GenePix Pro

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