The lysis effect of bull spermatozoa on gelatin substrate film methodical investigations.

Hoppe-Seyler's Zeitschrift für physiologische Chemie
V WendtH Fritz

Abstract

Proteolytic activity in the acrosomes of ejaculated bull spermatozoa was demonstrated using an autoradiographic film as a gelatin substrate. Incubation of the spermgelatin adducts at +37 degrees C and 94% humidity, which was kept constant by ventilating an incubator with water-saturated compressed air, yielded reproducible results. Gelatin depolymerisation started adjacent to the posterior segment of the acrosome within 30 to 60 s after application of individual spermatozoa to the substrate membrane and, finally, increased to a white circular digestion area enveloping the entire sperm head. The observed gelatinolysis seems to be mainly caused by acrosin, the trypsin-like acrosomal proteinase. This conclusion is supported by the positive correlation (r = +0.83, P is less than or equal to 0.01) found between the mean values of the lysis areas of individual spermatozoa on gelatin films and the acrosin activity of the sperm population measured with Bz-Arg-OEt as substrate after acidic extraction of the spermatozoa. In addition, prior saturation of the substrate layers with acrosin inhibitor (SSPI-I, II) from boar seminal plasma prevented the lysis reaction. Extraction of acrosin from the spermatozoa before application to the gelati...Continue Reading

Citations

Jan 1, 1983·Human Genetics·S Flörke-GerloffW Engel
Jan 1, 1993·Reproductive Toxicology·F H Comhaire
Mar 1, 1982·Acta Physiologica Scandinavica·O JohnsenC O Löfman
Nov 1, 1985·The American Journal of Anatomy·T T Huang, R Yanagimachi
Jul 1, 1977·The American Journal of Anatomy·P Gaddum-Rosse, R J Blandau
Aug 10, 1982·The Journal of Experimental Zoology·G Berruti, E Martegani
Feb 1, 1981·International Journal of Andrology·W B SchillJ Hammerstein
Dec 1, 1982·International Journal of Andrology·O JohnsenR Eliasson
Aug 5, 2008·Reproduction in Domestic Animals = Zuchthygiene·M DeppeR Sánchez

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