The M1 and M2 proteins of influenza A virus are important determinants in filamentous particle formation

Virology
P C RobertsR W Compans

Abstract

Influenza A virus is highly pleomorphic with particles exhibiting either spherical or filamentous morphology. The mechanisms behind this pleomorphism and its importance in viral pathogenesis are not clearly understood. We have observed that budding of filamentous influenza A/Udorn virus particles can be readily visualized by immunofluorescence microscopy. Filamentous particle formation was inhibited by treatment of cells with the anti-M2 14C2 antibody, but was not inhibited with the isotype identical 5C4 anti-M2 antibody or by anti-neuraminidase antibody. To further explore the viral determinants of filamentous particle formation, we investigated the morphology and growth characteristics of three variants of A/Udorn/72 virus, which had previously been selected for their resistance to growth inhibition by the 14C2 anti-M2 monoclonal antibody. Two of the variant viruses, 5A and 10A, contain single amino acid substitutions in the cytoplasmic domain of the M2 protein, whereas the 1A variant contains a single amino acid substitution in the viral matrix protein, M1. Variants 5A and 10A both were found to retain the filamentous particle phenotype found in the parental strain A/Udorn/72, and the production of filamentous virions by bot...Continue Reading

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Citations

Jul 26, 2008·Nature Nanotechnology·Yan GengDennis E Discher
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lesley J CalderPeter B Rosenthal
Nov 19, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Yufang YangRoys S Sundick
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Sep 26, 2012·Expert Review of Vaccines·Sang-Moo KangRichard W Compans
Feb 26, 2008·Expert Opinion on Biological Therapy·James A McSwiggen, Shaguna Seth

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