The M26 hotspot of Schizosaccharomyces pombe stimulates meiotic ectopic recombination and chromosomal rearrangements.

Genetics
J B Virgin, J P Bailey

Abstract

Homologous recombination is increased during meiosis between DNA sequences at the same chromosomal position (allelic recombination) and at different chromosomal positions (ectopic recombination). Recombination hotspots are important elements in controlling meiotic allelic recombination. We have used artificially dispersed copies of the ade6 gene in Schizosaccharomyces pombe to study hotspot activity in meiotic ectopic recombination. Ectopic recombination was reduced 10-1000-fold relative to allelic recombination, and was similar to the low frequency of ectopic recombination between naturally repeated sequences in S. pombe. The M26 hotspot was active in ectopic recombination in some, but not all, integration sites, with the same pattern of activity and inactivity in ectopic and allelic recombination. Crossing over in ectopic recombination, resulting in chromosomal rearrangements, was associated with 35-60% of recombination events and was stimulated 12-fold by M26. These results suggest overlap in the mechanisms of ectopic and allelic recombination and indicate that hotspots can stimulate chromosomal rearrangements.

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Citations

Aug 28, 2007·Trends in Cell Biology·Gareth A Cromie, Gerald R Smith
Jul 5, 2008·DNA Repair·Sylvia SteiningerSimone Moertl
Oct 19, 2004·Genetics·Michel BaurJuerg Kohli
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·L Davis, G R Smith
Jul 20, 2007·Journal of Virology·Aris KatzourakisMichael Tristem
Feb 3, 1999·Annual Review of Genetics·D Zickler, N Kleckner
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·A S Goldman, M Lichten

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