The m6A methyltransferase METTL3 aggravates the progression of nasopharyngeal carcinoma through inducing EMT by m6A-modified Snail mRNA

Minerva medica
Xiaofeng YuZhiwei Cao

Abstract

This study aims to elucidate the role of METTL3 in aggravating the progression of NPC through m6A modification on Snail and thus the stimulated EMT (epithelial-mesenchymal transition). Differential expressions of METTL3 in 48 paired NPC tissues and paracancerous tissues were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Its level in NPC patients with different clinical stages and metastatic states was examined. Prognostic potential of METTL3 in NPC patients was assessed by Kaplan-Meier method. After knockdown of METTL3, expression changes of Snail and EMT-related genes, as well as invasive and migratory abilities in SUNE-1 cells were detected. The interaction between Snail with METTL3 and IGF2BP2 was verified by RIP (RNA-Binding Protein Immunoprecipitation) assay. At last, the roles of METTL3/Snail regulatory loop in influencing EMT and metastasis of NPC were clarified. METTL3 was upregulated in NPC tissues than that of paracancerous ones. NPC patients with advanced stage or lymphatic metastasis expressed higher level of METTL3. Kaplan-Meier curves revealed that NPC patients expressing high level of METTL3 suffered worse prognosis. Knockdown of METTL3 downregulated protein levels of Snail and N-cadhe...Continue Reading

Citations

Feb 11, 2021·Journal of Experimental & Clinical Cancer Research : CR·Chenlong Song, Chongzhi Zhou
Apr 22, 2021·Biomarker Research·Yiqing CaiXin Wang
May 1, 2021·Journal of Experimental & Clinical Cancer Research : CR·Fenghua TanCan Guo
Mar 20, 2021·Molecular Therapy. Nucleic Acids·Hui LiDa Pang

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