Abstract
The expression of the myristoylated PKC substrate MARCKS is reduced in tumor-derived choroidal melanoma cells (OCM-1). We transfected the OCM-1 cells with MARCKS cDNA and we selected clones with stable overexpression of the protein. Tyrosine phosphorylation of paxillin, a biochemical marker of focal contact formation, was conserved upon serum starvation when MARCKS was overexpressed, while it was almost abolished in the control cells. Immunofluorescent labelling of paxillin and vinculin, another component of focal contact, revealed that these structures were conserved upon serum starvation when MARCKS was overexpressed but not in the control cells. Furthermore, the cell morphology was affected by the ectopic expression of MARCKS, leading to increased spreading and formation of membrane processes. These data suggest the involvement of MARCKS in cell spreading and focal contact formation.
References
Oct 1, 1992·The Journal of Cell Biology·J L Rodríguez FernándezA Ben-Ze'ev
Nov 27, 1992·Cell·A Aderem
Apr 16, 1992·Nature·J H HartwigA Aderem
Jul 31, 1991·Biochemical and Biophysical Research Communications·M Otsuka, H C Yang
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S L SimekN H Colburn
Aug 15, 1970·Nature·U K Laemmli
Jul 28, 1995·The Journal of Biological Chemistry·Z ZhuJ Li
Sep 1, 1995·The Journal of Experimental Medicine·L H Allen, A Aderem
Dec 1, 1994·Journal of Neurochemistry·S D RoséD M Byers
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·U GlückA Ben-Ze'ev
May 31, 1996·The Journal of Biological Chemistry·J LiZ Bao
Feb 1, 1996·Current Opinion in Cell Biology·S W Craig, R P Johnson
Sep 1, 1996·The Journal of Cell Biology·J M LewisM A Schwartz
Feb 1, 1997·Current Opinion in Cell Biology·K M Yamada, B Geiger
Aug 1, 1997·Current Biology : CB·M M MyatA Aderem
Citations
Jun 12, 2002·Biochemical and Biophysical Research Communications·Tetsuya NakamotoHisamaru Hirai
Mar 29, 2000·The International Journal of Biochemistry & Cell Biology·J J Ramsden
Jul 26, 2002·British Journal of Haematology·Arthur J ChuEvano Piasentin
Sep 22, 2006·Molecular Biology of the Cell·Jesse C GatlinKarl H Pfenninger
Jul 14, 2012·American Journal of Physiology. Renal Physiology·Abdel A AlliDouglas C Eaton
Jun 11, 2009·Journal of Cell Science·Adriana Estrada-BernalKarl H Pfenninger
Jul 19, 2011·Parasitology International·Anchalee TechasenPuangrat Yongvanit
Dec 13, 2006·Journal of Molecular and Cellular Cardiology·Maria C HeidkampAllen M Samarel
Jan 6, 2010·Cancer Science·Anchalee TechasenPuangrat Yongvanit
Mar 19, 2016·Journal of Neurochemistry·Lucas J SosaKarl H Pfenninger
Jul 12, 2013·PloS One·Chenglin WangLing Ye
Nov 3, 2004·Trends in Cell Biology·Fiona J Pixley, E Richard Stanley
Dec 13, 2005·Neurochemical Research·Sherry C MorashDavid M Byers
Feb 24, 2006·Cell Motility and the Cytoskeleton·Germain MazèresJean-Jacques Brustis
Mar 22, 2006·Molecular Carcinogenesis·Watcharin LoilomeMasanao Miwa
Mar 30, 2001·The Journal of Biological Chemistry·T JinJ Li
Jun 20, 2001·The Journal of Biological Chemistry·G Spizz, P J Blackshear
Apr 5, 2000·The Journal of Biological Chemistry·F WohnslandG Vergéres
Sep 24, 2009·Cancer Research·Johann MicallefAbhijit Guha
Aug 19, 2004·Journal of Cell Science·Marie-Hélène DisatnikThomas A Rando