The major nectar protein of Brassica rapa is a non-specific lipid transfer protein, BrLTP2.1, with strong antifungal activity

Journal of Experimental Botany
Anthony J SchmittClay J Carter

Abstract

Nectar is one of the key rewards mediating plant-mutualist interactions. In addition to sugars, nectars often contain many other compounds with important biological functions, including proteins. This study was undertaken to assess the proteinaceous content of Brassica rapa nectar. SDS-PAGE analysis of raw B. rapa nectar revealed the presence of ~10 proteins, with a major band at ~10 kDa. This major band was found to contain a non-specific lipid transfer protein encoded by B. rapa locus Bra028980 and subsequently termed BrLTP2.1. Sequence analysis of BrLTP2.1 predicted the presence of a signal peptide required for secretion from the cell, eight cysteines, and a mature molecular mass of 7.3 kDa. Constitutively expressed BrLTP2.1-GFP in Arabidopsis displayed accumulation patterns consistent with secretion from nectary cells. BrLTP2.1 was also found to have relatively high sequence similarity to non-specific lipid-transfer proteins with known functions in plant defense, including Arabidopsis DIR1. Heterologously expressed and purified BrLTP2.1 was extremely heat stable and bound strongly to saturated free fatty acids, but not methyl jasmonate. Recombinant BrLTP2.1 also had direct antimicrobial activity against an extensive range o...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Apr 25, 2002·Science's STKE : Signal Transduction Knowledge Environment·Simon DowlerDario R Alessi
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Jul 3, 2004·Trends in Plant Science·Clay Carter, Robert W Thornburg
Sep 10, 2004·Molecular Biology of the Cell·Nathalie BuhotJean-Pierre Blein
Dec 21, 2005·Die Naturwissenschaften·Clay CarterRobert Thornburg
Dec 14, 2006·Proceedings. Biological Sciences·Alexandra-Maria KleinTeja Tscharntke
Feb 9, 2008·Molecular Plant-microbe Interactions : MPMI·Jin-Yue SunAndré Laroche
Jun 17, 2008·Protein Science : a Publication of the Protein Society·Marie-Bernard LascombeThierry Prangé
Jan 16, 2009·The Plant Journal : for Cell and Molecular Biology·Marcia González-TeuberMartin Heil
Nov 1, 2004·FEMS Microbiology Ecology·Michael Brysch-Herzberg
Feb 12, 2010·Environmental Entomology·M A SasuA G Stephenson
Feb 23, 2010·PloS One·Venkatesan RadhikaMartin Heil
Feb 25, 2011·Trends in Plant Science·Martin Heil
Dec 17, 2011·The Plant Journal : for Cell and Molecular Biology·Yong Eui ChoiYun-Soo Kim
Dec 12, 2012·Proceedings. Biological Sciences·Rachel L VannetteTadashi Fukami
Apr 5, 2013·The Plant Journal : for Cell and Molecular Biology·Ricci L BenderClay J Carter
Jul 16, 2013·BMC Plant Biology·Pil Joon SeoSang-Gyu Kim
Apr 26, 2014·Frontiers in Microbiology·Ariane F LacerdaMaria F Grossi de Sa
Dec 31, 2014·Nature Methods·Jianyi YangYang Zhang

❮ Previous
Next ❯

Citations

Sep 18, 2020·Phytotherapy Research : PTR·Shilpi SrivastavaAtul Bhargava
Feb 28, 2021·Cellular and Molecular Life Sciences : CMLS·Ana Rita CavacoAndreia Figueiredo
Oct 22, 2021·Probiotics and Antimicrobial Proteins·Larissa Maximiano ResendeValdirene Moreira Gomes

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
fluorescence spectroscopy
fluorescence assay
size filtration
Dot
affinity purification

Software Mentioned

BLASTP
iTASSER
Geneious Tree Builder
PSORT
BLAST
Swiss
DeepView
PDBViewer
Analyst
CLUSTAL Omega

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.