The Major Origin of Seedless Grapes Is Associated with a Missense Mutation in the MADS-Box Gene VviAGL11

Plant Physiology
Carolina RoyoPablo Carbonell-Bejerano

Abstract

Seedlessness is greatly prized by consumers of fresh grapes. While stenospermocarpic seed abortion determined by the SEED DEVELOPMENT INHIBITOR (SDI) locus is the usual source of seedlessness in commercial grapevine (Vitis vinifera) cultivars, the underlying sdi mutation remains unknown. Here, we undertook an integrative approach to identify the causal mutation. Quantitative genetics and fine-mapping in two 'Crimson Seedless'-derived F1 mapping populations confirmed the major effect of the SDI locus and delimited the sdi mutation to a 323-kb region on chromosome 18. RNA-sequencing comparing seed traces of seedless and seeds of seeded F1 individuals identified processes triggered during sdi-determined seed abortion, including the activation of salicylic acid-dependent autoimmunity. The RNA-sequencing data set was investigated for candidate genes, and while no evidence for causal cis-acting regulatory mutations was detected, deleterious nucleotide changes in coding sequences of the seedless haplotype were predicted in two genes within the sdi fine-mapping interval. Targeted resequencing of the two genes in a collection of 124 grapevine cultivars showed that only the point variation causing the arginine-197-to-leucine substitution...Continue Reading

Citations

Mar 19, 2019·Frontiers in Plant Science·Guo-Qing SongVladimir Orbovic
Apr 6, 2019·Frontiers in Plant Science·Fabio PalumboGianni Barcaccia
Nov 24, 2019·Proceedings of the National Academy of Sciences of the United States of America·Juan-José RipollAdrienne H K Roeder
Mar 14, 2021·BMC Plant Biology·Laura CostantiniMaria Stella Grando
Mar 30, 2021·Plant Science : an International Journal of Experimental Plant Biology·Lalla Hasna ZinelabidineJavier Tello
Dec 23, 2021·Nature Communications·Gabriele MagrisMichele Morgante

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