Mar 19, 1976

The major sialoglycoprotein of the human erythrocyte membrane. Release with a non-ionic detergent and purification

Biochimica Et Biophysica Acta
L LiljasS Hjertén

Abstract

The major sialoglycoprotein of the human erythrocyte membrane has been selectively released by the non-ionic detergent Tween 20 and further purified in detergent-free buffers by hydroxyapatite chromatography and, finally, by hydrophobic interaction chromatography on pentyl-Sepharose. The purified glycoprotein shows one main zone, PAS-1, and up to three minor zones after staining both for protein and carbohydrate in polyacrylamide gel electrophoresis in the presence of dodecyl sulfate. The relative staining intensities are concentration dependent. When the purified glycoprotein has been heated to 100 degrees C in dodecyl sulfate, more stain appears in the most rapid zone, PAS-2, and less in the slower zones, indicating a disaggregation of oligomeric forms of this glycoprotein, including a dimer, PAS-1.

Mentioned in this Paper

Buffers
Tweens
Hexoses
Glycoproteins
Chromatography
Neoglycoproteins
Purification Aspects
Dimer
Sepharose
Sialic Acids

About this Paper

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