The major soluble tubulins are found in mega dalton (MDa) fractions in fully-grown oocytes and eggs but not in brain of the frog, Rana pipiens

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
T Wang, C A Lessman

Abstract

During progesterone-induced oocyte maturation, the cell is converted from prophase I to metaphase II, which is known to involve microtubule changes. We hypothesize that progesterone affects the polymeric state of tubulins, isoform dynamics, as well as tubulin synthesis and degradation. To test the former, leopard frog (Rana pipiens) oocytes were treated with progesterone, homogenized and centrifuged at 16,000 x g. The resulting pellet and supernatant fractions were analyzed using immunoblots probed with anti-alpha-tubulin antibody DM1A and anti-beta-tubulin antibody DM1B. The results indicate that the majority of tubulins are in supernatant fractions (soluble cellular component) and further indicate changes in size of pelletable tubulins with time post-progesterone treatment. To characterize the supernatant tubulins, Superose-6b columns were used to fractionate 16,000 x g supernatant materials. The results indicate tubulins in fractions of up to 5 MDa (equivalent in size to c. 50 tubulin dimers or 5,000 kDa) predominate, while tubulin dimers and monomers of 110 kDa and 55 kDa, respectively, are relatively minor components. The tubulin in MDa fraction pools in the oocyte soluble component seem to be tissue specific, since the re...Continue Reading

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Citations

Feb 5, 2008·Gene Expression Patterns : GEP·Jianxiong Liu, Charles A Lessman
Sep 11, 2001·Molecular Reproduction and Development·C A Lessman, H Kim
Feb 13, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jianxiong Liu, Charles A Lessman

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