Abstract
Members of the BCL-2 gene family are well known for their role in the pathogenesis of B-cell lymphomas in humans and in mouse models. A recent report that knockout mice deficient for the proapoptotic BCL-2 family member gene BAD frequently develop B-cell lymphomas prompted us to analyze a large collection of human B-cell lymphomas for inactivating mutations in the BAD gene. All 3 exons of the BAD gene were amplified and directly sequenced. The 81 lymphomas analyzed included 16 cases of B-cell chronic lymphocytic leukemia, 11 mantle-cell lymphomas, 10 follicular lymphomas, 7 MALT lymphomas, 8 Burkitt's lymphoma cell lines, 3 cell lines of multiple myeloma, 15 cases and 4 cell lines of diffuse large B-cell lymphoma and 7 Hodgkin's lymphoma lines. No mutations were found in any of the cases. We conclude that mutations in the BAD gene do not play a role in the pathogenesis of the major subtypes of human B-cell lymphomas.
References
Apr 7, 1989·Cell·T J McDonnellS J Korsmeyer
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M L Cleary, J Sklar
Nov 30, 1984·Science·Y TsujimotoC M Croce
Nov 15, 1996·Cell·J ZhaS J Korsmeyer
Nov 5, 1997·Cell·S R DattaM E Greenberg
Oct 24, 1997·Science·L del PesoG Nuñez
Oct 12, 1999·Leukemia·L XerriJ Plumas
Feb 15, 2001·Cell Death and Differentiation·S J KorsmeyerP H Schlesinger
Apr 28, 2001·Science·M C WeiS J Korsmeyer
Oct 5, 2001·Molecular Cell·E H ChengS J Korsmeyer
Sep 21, 2002·Cancer Cell·Anthony LetaiStanley J Korsmeyer
Oct 9, 2002·European Journal of Haematology·Helena SpetsHelena Jernberg-Wiklund
Nov 5, 2002·Annual Review of Immunology·Vanessa S Marsden, Andreas Strasser
Jan 21, 2003·Genes & Development·Sandra S ZinkelStanley J Korsmeyer
Jul 23, 2003·Genes, Chromosomes & Cancer·Vincenzo ArenaLuigi Maria Larocca
Jul 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ann M RangerStanley J Korsmeyer
Sep 27, 2003·Leukemia·J L GarcíaJ F San Miguel
Jan 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Carsten SchwaenenPeter Lichter
Mar 23, 2004·Carcinogenesis·Jong Woo LeeSug Hyung Lee
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alexander EgleSuzanne Cory
May 20, 2004·Cancer Research·Celine PompeiaWilliam L Farrar
Jun 17, 2004·British Journal of Haematology·Roman K ThomasRoland Schmitz
Feb 19, 2005·Blood·Fanny Rubio-MoscardoJose A Martinez-Climent
May 12, 2005·Blood·Hiroyuki TagawaMasao Seto
Sep 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Amelia CimminoCarlo M Croce