PMID: 7528769Jan 1, 1995Paper

The majority of postselection CD4+ single-positive thymocytes requires the thymus to produce long-lived, functional T cells

The Journal of Experimental Medicine
R Dyall, J Nikolic-Zugić

Abstract

We have previously isolated, and characterized in vitro, two subsets of CD4hi T cell receptor (TCR)hi single positive (SP) thymocytes: CD8- and CD8lo. In this report, we have analyzed phenotypic, functional, and developmental characteristics of these "late" CD4hi SP thymocyte subsets. The TCRhi phenotype and the elimination of T cells expressing TCR V beta segments reactive with endogenous mouse mammary tumor virus (MMTV) products suggested that both subsets had undergone positive and negative selection. CD8-4hi thymocytes were functional, as judged by their ability to: (a) induce lethal graft versus host disease (GVHD); (b) survive and expand in peripheral lymphoid organs; and (c) proliferate, rather than undergo apoptosis, in response to in vitro TCR cross-linking. By contrast, CD8lo4hi cells could not induce GVHD, were unable to expand (and perhaps even survive) in peripheral organs and underwent apoptosis upon TCR cross-linking. However, when reintroduced into the thymus, these cells matured into functional, long-lived CD8-4hi lymphocytes. These results document an obligatory requirement for the thymic microenvironment in the final maturation of the majority of CD4hi SP postselection thymocytes, and demonstrate the existenc...Continue Reading

References

Apr 1, 1992·Current Opinion in Immunology·K Shortman
Sep 1, 1992·European Journal of Immunology·W SwatH von Boehmer
Mar 1, 1992·The Journal of Experimental Medicine·A BendelacR H Schwartz
Feb 7, 1991·Nature·C Janeway
Jan 1, 1990·International Immunology·J Nikolić-Zugić, M J Bevan
Jan 1, 1990·International Immunology·K A Kelly, R Scollay
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M EgertonK Shortman
Jan 1, 1990·Annual Review of Immunology·H von Boehmer
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C Penit, S Ezine
Apr 1, 1989·European Journal of Immunology·J Nikolić-ZugićM J Bevan
Jan 1, 1985·Immunogenetics·J LesleyR Hyman
Dec 1, 1985·The Journal of Experimental Medicine·J Sprent, M Schaefer
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A L CrumpH Cantor
Mar 1, 1993·European Journal of Immunology·W SwatP Kisielow
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T CromptonH R MacDonald

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Citations

Nov 9, 2000·European Journal of Immunology·H LeendersD Mathis
Sep 29, 1999·Immunology Today·G AndersonE J Jenkinson
Apr 1, 1996·Current Opinion in Immunology·C J Guidos
Oct 9, 2013·Immunology and Cell Biology·Rong JinQing Ge
Jan 1, 1996·Annual Review of Immunology·G AndersonE J Jenkinson
Feb 13, 2007·Annual Review of Immunology·Howard T Petrie, Juan Carlos Zúñiga-Pflücker
Nov 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Juan LiWei-Feng Chen
Sep 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lisa K McNeilKristin A Hogquist
Jan 20, 1997·The Journal of Experimental Medicine·H Kishimoto, J Sprent
Jun 1, 2002·International Immunology·Chiguang FengPaul E Love
Feb 1, 1997·European Journal of Immunology·L V VasovićJ Nikolić-Zugic
Aug 1, 1997·European Journal of Immunology·M J GaborR Scollay
May 1, 1997·European Journal of Immunology·U BommhardtR Zamoyska
Feb 5, 1999·European Journal of Immunology·R DyallJ Nikolić-Zugić
Jun 26, 1999·European Journal of Immunology·G Hernández-HoyosG W Butcher
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ilhem MessaoudiJanko Nikolich-Zugich
May 31, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thiago DetanicoLawrence J Wysocki
May 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva JiménezAlberto Varas
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K J HareG Anderson
Sep 13, 2003·Immunological Reviews·Jason GillRichard Boyd

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