PMID: 2104856Jan 1, 1990Paper

The majority of type 1 plasminogen activator inhibitor associated with cultured human endothelial cells is located under the cells and is accessible to solution-phase tissue-type plasminogen activator

The Journal of Cell Biology
R R SchleefD J Loskutoff

Abstract

The interactions between exogenously added tissue-type plasminogen activator (t-PA) and the active form of type 1 plasminogen activator inhibitor (PAI-1) produced by and present in cultured human umbilical vein endothelial cells (HUVECs) were investigated. Immunoblotting analysis of the conditioned media obtained from monolayers of HUVECs treated with increasing concentrations of t-PA (less than or equal to 10 micrograms/ml) revealed a dose-dependent formation of both t-PA/PAI-1 complexes, and of a 42,000-Mr cleaved or modified form of the inhibitor. Immunoradiometric assays indicated that t-PA treatment resulted in a fourfold increase in PAI-1 antigen present in the conditioned media. This increase did not result from the release of PAI-1 from intracellular stores, but rather reflected a t-PA-dependent decrease in the PAI-1 content of the Triton X-100 insoluble extracellular matrix (ECM). Although the rate of t-PA-mediated release of PAI-1 was increased by the removal of the monolayer, similar quantities of PAI-1 were removed in the presence or absence of the cells. These results suggest that the cells only represent a semipermeable barrier between ECM-associated PAI-1 and exogenous t-PA. Treatment of HUVECs with t-PA (1 micro...Continue Reading

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Citations

Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·D SeiffertD J Loskutoff
May 16, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·H FukaoO Matsuo
Sep 17, 1999·Atherosclerosis·F Pérez-JiménezJ M Ordovás
Jan 1, 1997·Progress in Cardiovascular Diseases·H R Lijnen, D Collen
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Oct 1, 1997·Trends in Cardiovascular Medicine·V Ellis
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Nov 5, 2002·Journal of Clinical Pharmacology·James P TsikourisGary E Meyerrose
May 30, 2008·Thrombosis Research·Helén BrogrenSverker Jern
Feb 24, 2007·Biomaterials·Alison P McGuigan, Michael V Sefton
Oct 1, 1991·Experimental Cell Research·M AumailleyW Risau
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Aug 13, 2013·Acta Biomaterialia·Jacob Ceccarelli, Andrew J Putnam
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Feb 18, 2010·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Chikako IshidaOsamu Matsuo

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