PMID: 7545033May 1, 1995Paper

The majority of yeast UPF1 co-localizes with polyribosomes in the cytoplasm

Molecular Biology of the Cell
A L AtkinMichael R Culbertson

Abstract

In Saccharomyces cerevisiae the UPF1 protein is required for nonsense-mediated mRNA decay, the accelerated turnover of mRNAs containing a nonsense mutation. Several lines of evidence suggest that translation plays an important role in the mechanism of nonsense mRNA decay, including a previous report that nonsense mRNAs assemble in polyribosomes. In this study we show that UPF1 and ribosomal protein L1 co-localize in the cytoplasm and that UPF1 co-sediments with polyribosomes. To detect UPF1, three copies of the influenza hemagglutinin epitope were placed at the C-terminus. The tagged protein, UPF1-3EP, retains 86% (+/- 5%) of function. Using immunological detection, we found that UPF1-3EP is primarily cytoplasmic and was not detected either in the nucleus or in the mitochondrion. UPF1-3EP and L1 co-distributed with polyribosomes fractionated in a 7-47% sucrose gradient. The sucrose sedimentation profiles for UPF1-3EP and L1 exhibited similar changes using three different sets of conditions that altered the polyribosome profile. When polyribosomes were disaggregated, UPF1-3EP and L1 accumulated in fractions coincident with 80S ribosomal particles. These results suggest that UPF1-3EP associates with polyribosomes. L3 and S3 mRNAs...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R Losson, F Lacroute
Dec 1, 1992·Trends in Biochemical Sciences·E V Koonin
Jul 1, 1992·The Journal of Cell Biology·D M RoofM D Rose
Mar 5, 1987·Journal of Molecular Biology·M D MendenhallM R Culbertson
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 24, 1981·Science·T F DonahueG R Fink
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H M Fried, J R Warner
Aug 13, 1993·Cell·A B Sachs
Oct 1, 1993·Genes & Development·R Pulak, P Anderson
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·F HeA Jacobson
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P BelgraderL E Maquat

❮ Previous
Next ❯

Citations

Aug 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K CzaplinskiS W Peltz
Sep 16, 2011·Current Genetics·Rafael Deliz-AguirreBessie W Kebaara
Oct 5, 2013·Trends in Genetics : TIG·Bijoyita Roy, Allan Jacobson
Feb 25, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·R L SlaytonM C Willing
May 26, 2006·Nature Reviews. Molecular Cell Biology·Nadia AmraniAllan Jacobson
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·B S Lee, M R Culbertson
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·H A PerlickH C Dietz
Jun 21, 2013·Journal of Virology·Anna Kristina P SerquiñaHeinrich G Göttlinger
Jul 21, 2001·Molecular and Cellular Biology·K S Rajavel, E F Neufeld
Oct 5, 2001·Molecular and Cellular Biology·A T BondA Jacobson
Feb 3, 1999·Annual Review of Genetics·J W Jarvik, C A Telmer
Mar 29, 2008·RNA·Lara AjamianAndrew J Mouland
May 26, 2010·Genetics·Arun DakshinamurthyDavid J Garfinkel
Nov 8, 2013·Nucleic Acids Research·Clarivel LasaldeCarlos I González
Jul 31, 2013·Nucleic Acids Research·Kinga P OrlowskaAndrzej Dziembowski
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·X SunL E Maquat
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·M A MacMorrisT Blumenthal
Oct 24, 2001·Trends in Genetics : TIG·S E Mango
Jun 2, 2005·Reviews of Physiology, Biochemistry and Pharmacology·S RospertY Dubaquié
Apr 1, 1997·Current Opinion in Genetics & Development·M WickensR J Jackson
Dec 16, 1998·The Journal of Cell Biology·M AlbertiniG Blobel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.