The Makes Caterpillars Floppy (MCF)-Like Domain of Vibrio vulnificus Induces Mitochondrion-Mediated Apoptosis

Infection and Immunity
Shivangi AgarwalKarla J F Satchell

Abstract

The multifunctional-autoprocessing repeats-in-toxin (MARTXVv) toxin of Vibrio vulnificus plays a significant role in the pathogenesis of this bacterium through delivery of up to five effector domains to the host cells. Previous studies have established that the MARTXVv toxin is linked to V. vulnificus dependent induction of apoptosis, but the region of the large multifunction protein essential for this activity was not previously identified. Recently, we showed that the Makes Caterpillar Floppy-like MARTX effector domain (MCFVv) is an autoproteolytic cysteine protease that induces rounding of various cell types. In this study, we demonstrate that cell rounding induced by MCFVv is coupled to reduced metabolic rate and inhibition of cellular proliferation. Moreover, delivery of MCFVv into host cells either as a fusion to the N-terminal fragment of anthrax toxin lethal factor or when naturally delivered as a V. vulnificus MARTX toxin led to loss of mitochondrial membrane potential, release of cytochrome c, activation of Bax and Bak, and processing of caspases and poly-(ADP-ribose) polymerase (PARP-γ). These studies specifically link the MCFVv effector domain to induction of the intrinsic apoptosis pathway by V. vulnificus.

References

Jul 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·P J DabornR H Ffrench-Constant
Sep 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stefan HippenstielNorbert Suttorp
Dec 24, 2002·Infection and Immunity·Takashige KashimotoNobuyuki Susa
Apr 18, 2003·Cell Death and Differentiation·C FiorentiniA Fabbri
Dec 19, 2003·FEMS Microbiology Letters·Nicholas R WaterfieldRichard H ffrench-Constant
Apr 10, 2004·The Journal of Biological Chemistry·Alam Nur-E-KamalLeroy F Liu
Jun 26, 2007·Analytical Biochemistry·Nicole Maria SeibelWolfgang Hampe
Nov 17, 2007·Cellular Microbiology·Young Ran KimJoon Haeng Rhee
May 20, 2008·Cell·Lai WangXiaodong Wang
Mar 4, 2009·Infection and Immunity·Melissa K Jones, James D Oliver
Jun 17, 2009·Cell Death and Differentiation·S P Cullen, S J Martin
Jul 18, 2009·PLoS Pathogens·Isabella VlisidouWill Wood
Nov 16, 2010·Applied and Environmental Microbiology·Francisco J RoigCarmen Amaro
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jayme S KwakKarla J F Satchell
Feb 22, 2011·Apoptosis : an International Journal on Programmed Cell Death·Ning GaoXianglin Shi
Mar 4, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nicholas A Daniels
Jun 7, 2011·Annual Review of Microbiology·Karla J F Satchell
Jul 13, 2011·Methods in Molecular Biology·Giles Cory
Sep 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Prashant JainSteven R Blanke
Dec 3, 2011·Molecular Medicine Reports·Jiachun SunHong Shen
Dec 12, 2012·The Journal of Infectious Diseases·Young Ran KimJoon Haeng Rhee
Jun 5, 2013·Cold Spring Harbor Perspectives in Biology·Amanda B ParrishSally Kornbluth
Jun 9, 2015·Nature Communications·Irena AnticKarla J F Satchell
Jul 3, 2015·The Journal of Infectious Diseases·Bo A KimYoung Ran Kim

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Citations

May 2, 2017·Biochemistry·Marco BiancucciKarla J F Satchell
Nov 28, 2018·The Journal of Biological Chemistry·Xenia BogdanovicThomas Jank
Oct 6, 2018·The Journal of Infectious Diseases·Hannah E Gavin, Karla J F Satchell
Jun 21, 2017·BMC Microbiology·Hannah E Gavin, Karla J F Satchell
Nov 9, 2017·Molecular Microbiology·Patrick J Woida, Karla J F Satchell
Aug 5, 2017·Frontiers in Cellular and Infection Microbiology·Celia MurcianoCarmen Amaro
Dec 6, 2018·Toxins·Byoung Sik Kim
Sep 10, 2020·Journal of Bacteriology·Alfa Herrera, Karla J F Satchell

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