The malignant brain tumor repeats of human SCML2 bind to peptides containing monomethylated lysine.

Journal of Molecular Biology
Clara M SantiveriMark Bycroft

Abstract

SCML2 (sex comb on midleg-like 2) is a constituent of the Polycomb repressive complex 1, a large multiprotein assembly required for the repression of developmental control genes. It contains two MBT (malignant brain tumor) repeats; the MBT is a protein module structurally similar to domains that bind to methylated histones. We have used NMR spectroscopy to examine the binding specificity of these repeats. Our data show that they preferentially bind histone peptides monomethylated at lysine residues with no apparent sequence specificity. The crystal structure of the complex between the protein and monomethyllysine reveals that the modified amino acid binds to an aromatic rich pocket at one end of the beta-barrel of the second repeat.

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Citations

Apr 2, 2011·The Journal of Biological Chemistry·Zhanxin Wang, Dinshaw J Patel
Feb 24, 2009·Nucleic Acids Research·Yahong GuoJinrong Min
Oct 21, 2009·PloS One·Jitka EryilmazJinrong Min
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May 7, 2016·Genes & Development·Felice FreyJürg Müller
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Apr 15, 2011·The Journal of Biological Chemistry·Nataliya NadySirano Dhe-Paganon
Apr 15, 2014·The Journal of Biological Chemistry·Irina Bezsonova
Oct 18, 2020·Nature Communications·Alexandra StützerHenning Urlaub

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