The maltoporin of Salmonella typhimurium: sequence and folding model
Abstract
The sequence of the lamB gene from Salmonella typhimurium was determined. It encodes the precursor to the LamB protein from S. typhimurium (pre-LamBS.t.; 452 residues) which presents extensive homologies with the pre-LamB protein from Escherichia coli (pre-LamBE.c.; 446 residues). The first third of pre-LamBS.t. is the most conserved, with 4% changes and strict identity between the signal peptides. The last two-third contains five "variable" segments where more than 50% of the residues are changed with respect to LamBE.c.. The three first variable segments are 8 to 14 residues long and contain only substitutions, while the two more distal ones are 24 and 29 residues long and also include insertions and deletions. It is remarkable that the variable segments correspond essentially to regions predicted to be extramembranous loops on our 2D folding model for LamBE.c.; they alternate with conserved predicted transmembranous segments. Four of the variable regions were predicted to be cell-surface-exposed loops on the basis of genetic and immunological data, while one of them (region II) was predicted to be periplasmic on the sole basis of folding rules. The LamB protein from S. typhimurium can substitute for the LamB protein from E. ...Continue Reading
References
Isolation of different bacteriophages using the LamB protein for adsorption on Escherichia coli K-12
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