The mammalian homolog of suppressor-of-white-apricot regulates alternative mRNA splicing of CD45 exon 4 and fibronectin IIICS.

The Journal of Biological Chemistry
M SarkissianR Lafyatis

Abstract

We have previously described human (HsSWAP) and mouse (MmSWAP) homologs to the Drosophila alternative splicing regulator suppressor-of-white-apricot (su(wa) or DmSWAP). DmSWAP was formally defined as an alternative splicing regulator by studies showing that it autoregulates splicing of its own pre-mRNA. We report here that mammalian SWAP regulates its own splicing, and also the splicing of fibronectin and CD45. Using an in vivo system of cell transfection, mammalian SWAP regulated 5' splice site selection in splicing of its own second intron. SWAP enhanced splicing to the distal 5' splice site, whereas the SR protein ASF/SF2 enhanced splicing to the proximal site. SWAP also regulated alternative splicing of the fibronectin IIICS region by promoting exclusion of the entire IIICS region. In contrast, ASF/SF2 stimulated inclusion of the entire IIICS region. Finally, SWAP regulated splicing of CD45 exon 4, promoting exclusion of this exon, an effect also seen with ASF/SF2. Experiments using SWAP deletion mutants showed that splicing regulation of the fibronectin IIICS region and CD45 exon 4 requires a region including a carboxyl-terminal arginine/serine (R/S)-rich motif. Since R/S motifs of various splicing proteins have been shown...Continue Reading

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Citations

Mar 26, 1999·European Journal of Immunology·R LemaireR Lafyatis
Jul 12, 2002·Journal of Cellular Biochemistry·Bruce A KuoPamela A Norton
May 15, 2002·Brain Research. Molecular Brain Research·Meltem Cevik ArikanAthena Andreadis
Aug 28, 2003·Brain Research. Molecular Brain Research·Karen LiAthena Andreadis
Jan 16, 1999·Brain Research. Molecular Brain Research·S StammD M Helfman
Dec 11, 1999·Brain Research. Brain Research Protocols·O StossS Stamm
Jan 26, 2000·Journal of Neurochemistry·Q S GaoA Andreadis
Nov 5, 2002·Annual Review of Immunology·Michelle L HermistonArthur Weiss
Jul 24, 2007·Blood·Stephen M JacksonJ Donald Capra
Oct 1, 2015·Nucleic Acids Research·Angela CrisciAngela Krämer
Aug 6, 2008·The Journal of Pathology·E S WhiteA F Muro
Aug 1, 1997·American Journal of Human Genetics·T A Cooper, W Mattox
Jul 5, 2016·Frontiers of Medicine·Yanfei WangZhigang Xu
Apr 18, 2001·The Journal of Biological Chemistry·K W Lynch, A Weiss
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G B ten DamG R Screaton
Jul 20, 2001·Molecular and Cellular Neurosciences·A M HartmannS Stamm

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