The mammalian homolog of yeast Sec13p is enriched in the intermediate compartment and is essential for protein transport from the endoplasmic reticulum to the Golgi apparatus.

Molecular and Cellular Biology
B L TangW Hong

Abstract

The role of COPII components in endoplasmic reticulum (ER)-Golgi transport, first identified in the yeast Saccharomyces cerevisiae, has yet to be fully characterized in higher eukaryotes. A human cDNA whose predicted amino acid sequence showed 70% similarity to the yeast Sec13p has previously been cloned. Antibodies raised against the human SEC13 protein (mSEC13) recognized a cellular protein of 35 kDa in both the soluble and membrane fractions. Like the yeast Sec13p, mSEC13 exist in the cytosol in both monomeric and higher-molecular-weight forms. Immunofluorescence microscopy localized mSEC13 to the characteristic spotty ER-Golgi intermediate compartment (ERGIC) in cells of all species examined, where it colocalized well with the KDEL receptor, an ERGIC marker, at 15 degrees C. Immunoelectron microscopy also localized mSEC13 to membrane structures close to the Golgi apparatus. mSEC13 is essential for ER-to-Golgi transport, since both the His6-tagged mSEC13 recombinant protein and the affinity-purified mSEC13 antibody inhibited the transport of restrictive temperature-arrested vesicular stomatitis virus G protein from the ER to the Golgi apparatus in a semi-intact cell assay. Moreover, cytosol immunodepleted of mSEC13 could no ...Continue Reading

References

Jan 30, 1992·Nature·J E Rothman, L Orci
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L OrciR Schekman
Jan 1, 1987·Annual Review of Biochemistry·S R Pfeffer, J E Rothman
Nov 1, 1987·The Journal of Cell Biology·J SarasteM G Farquhar
Aug 1, 1995·Current Opinion in Cell Biology·N R Salama, R W Schekman
Aug 1, 1994·Current Opinion in Cell Biology·T E Kreis, R Pepperkok
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L OrciJ E Rothman
Apr 1, 1993·The Journal of Cell Biology·A OprinsJ W Slot
May 1, 1993·The Journal of Cell Biology·J B HelmsJ E Rothman
Apr 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·W Hong, B L Tang

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Citations

Nov 3, 2009·FEBS Letters·Effrosyni Papanikou, Benjamin S Glick
May 16, 1998·The Journal of Cell Biology·M AridorW E Balch
Jun 16, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Maria Rita De GiorgioJonny St-Amand
Aug 19, 2007·Seminars in Cell & Developmental Biology·Semra J Kirk, Theresa H Ward
Jun 28, 2005·Biochimica Et Biophysica Acta·Bor Luen TangWanjin Hong
Aug 26, 1998·Biochimica Et Biophysica Acta·E C GaynorS D Emr
Aug 18, 2009·Biochemical and Biophysical Research Communications·Anders Lade Nielsen
Mar 13, 1999·The Journal of Biological Chemistry·A PaganoJ P Paccaud
Jun 8, 1999·Molecular Biology of the Cell·J FüllekrugT Nilsson
Jan 26, 2002·Molecular Biology of the Cell·Hisao NagayaHideo Kanoh
May 18, 2004·Molecular Biology of the Cell·Isabelle LoïodiceValérie Doye
Sep 12, 2000·Molecular Biology of the Cell·A T Hammond, B S Glick
Jun 1, 2010·Nature Methods·Kristine SchauerBruno Goud
Sep 19, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Silke StertzGeorg Kochs
Sep 23, 2006·Nature Reviews. Molecular Cell Biology·Cemal GürkanWilliam E Balch
Oct 3, 2002·Nature Cell Biology·Brooke J BevisBenjamin S Glick
Jun 7, 2012·Cellular and Molecular Life Sciences : CMLS·Sidney Yu, Yongheng Liang
Sep 29, 2019·Plant & Cell Physiology·Aleksandra SulkowskaJoanna Kufel
Jul 21, 1998·The Journal of Biological Chemistry·C J Fernández, G Warren
Aug 8, 2001·The Journal of Biological Chemistry·B L TangW Hong
Apr 18, 1998·The Journal of Biological Chemistry·B L TangW Hong

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