The manganese-responsive regulator MntR represses transcription of a predicted ZIP family metal ion transporter in Corynebacterium glutamicum

FEMS Microbiology Letters
Meike Baumgart, Julia Frunzke

Abstract

Manganese is an important trace element required as an enzyme cofactor and for protection against oxidative stress. In this study, we characterized the DtxR-type transcriptional regulator MntR (cg0741) of Corynebacterium glutamicum ATCC 13032 as a manganese-dependent repressor of the predicted ZIP family metal transporter Cg1623. Comparative transcriptome analysis of a ΔmntR strain and the wild type led to the identification of cg1623 as potential target gene of MntR which was about 50-fold upregulated when cells were grown in glucose minimal medium. Using electrophoretic mobility shift assays, a conserved 18 bp inverted repeat (TGTTCAATGCGTTGAACA) was identified as binding motif of MntR in the cg1623 promoter and confirmed by mutational analysis. Promoter fusion of Pcg1623 to eyfp confirmed that the MntR-dependent repression is only abolished in the absence of manganese. However, neither deletion of mntR nor cg1623 resulted in a significant growth phenotype in comparison to the wild type--strongly suggesting the presence of further manganese uptake and efflux systems in C. glutamicum. The control of cg1623 by the DtxR-type regulator MntR represents the first example of a predicted ZIP family protein that is regulated in a mang...Continue Reading

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J BoydJ R Murphy
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Oct 1, 1994·Molecular Microbiology·X TaoJ R Murphy
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·N GrotzD Eide
Feb 22, 2000·The Journal of Biological Chemistry·L A Gaither, D J Eide
Apr 5, 2000·Biochimica Et Biophysica Acta·M L Guerinot
Apr 3, 2001·Current Opinion in Microbiology·K Hantke
Jan 16, 2002·Journal of Bacteriology·Gregor GrassChristopher Rensing
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michelle M SpieringMichael A Marletta
Apr 5, 2003·Molecular Microbiology·Emmanuel Guedon, John D Helmann
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Sep 2, 2003·Journal of Biotechnology·Oliver Kirchner, Andreas Tauch
Jun 25, 2004·Nucleic Acids Research·Burkhard RostJinfeng Liu
Dec 21, 2004·Nucleic Acids Research·Hiroyuki Ogata, Jean-Michel Claverie
Feb 18, 2005·Journal of Bacteriology·Gregor GrassChristopher Rensing
Jul 7, 2005·Genome Research·Eric J AlmAdam P Arkin
Dec 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·J Alejandro D'AquinoDagmar Ringe
Apr 6, 2006·Journal of Bacteriology·Julia Wennerhold, Michael Bott
Nov 23, 2006·Journal of Molecular Biology·Mark A DeWittArthur Glasfeld
Jan 9, 2010·BMC Genomics·Jasmin SchröderAndreas Tauch
Mar 13, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Nadine Taudte, Gregor Grass
Nov 5, 2011·Nucleic Acids Research·Ivica LetunicPeer Bork
Apr 19, 2013·Metal Ions in Life Sciences·Simon AndrewsPierre Cornelis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.