The Manipulation of RNA-Guided Nucleic Acid Cleavage with Ninhydrin Chemistry

Advanced Science
Shao-Ru WangXiang Zhou

Abstract

CRISPR (clustered regularly interspaced short palindromic repeats) systems have been established as valuable genome-editing tools. Controlling CRISPR systems has high biological significance and this field has garnered intense interest. There is a considerable need for simple approaches with no need for protein engineering. The CRISPR systems usually require a guide RNA (gRNA) moiety to recruit and direct the nuclease complexes. In this respect, the ninhydrin (1,2,3-indantrione monohydrate) seems to have considerable potential, as yet unexploited, for modifying gRNA. In this study, ninhydrin chemistry is explored for reversible postsynthetic modification of gRNA molecules. It is further shown that ninhydrin chemistry is efficient in modulating two important CRISPR systems. Thus, ninhydrin chemistry exhibits potential applications in future chemical biology studies.

References

Nov 21, 1967·Biochimica Et Biophysica Acta·M Y Feldman
Jan 17, 1968·Journal of the American Chemical Society·R Shapiro, S C Agarwal
Sep 1, 1966·Biochemistry·R Shapiro, J Hachmann
Oct 1, 1954·Biochimica Et Biophysica Acta·H FRAENKEL-CONRAT
Aug 1, 1958·Biochimica Et Biophysica Acta·M STAEHELIN
Feb 1, 1961·Journal of Molecular Biology·L GROSSMANW S ALLISON
Jan 12, 2005·Angewandte Chemie·Samit ShahSimon H Friedman
Mar 24, 2007·Science·Rodolphe BarrangouPhilippe Horvath
Jan 12, 2008·Chemical Communications : Chem Comm·Douglas D YoungAlexander Deiters
Nov 5, 2010·Nature·Josiane E GarneauSylvain Moineau
Apr 2, 2011·Nature·Elitza DeltchevaEmmanuelle Charpentier
Aug 5, 2011·Nucleic Acids Research·Rimantas SapranauskasVirginijus Siksnys
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Giedrius GasiunasVirginijus Siksnys
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 5, 2013·Science·Prashant MaliGeorge M Church
Feb 18, 2014·Cell·Hiroshi NishimasuOsamu Nureki
Feb 11, 2015·Nature Chemical Biology·Lauren R Polstein, Charles A Gersbach
Feb 19, 2015·Nature Biotechnology·Lukas E DowScott W Lowe
Apr 8, 2015·Nature Chemical Biology·Kevin M DavisDavid R Liu
Nov 26, 2015·Molecular Cell·Sergey ShmakovEugene V Koonin
Dec 3, 2015·Science·Ian M SlaymakerFeng Zhang
Dec 17, 2015·ACS Chemical Biology·Xiaoyan WangYu Xiang
Aug 25, 2016·Angewandte Chemie·Piyush K JainSangeeta N Bhatia
Sep 27, 2016·Nature·Alexandra East-SeletskyJennifer A Doudna
Oct 22, 2016·Nature Chemistry·Ji LuoAlexander Deiters
Apr 15, 2017·Science·Jonathan S GootenbergFeng Zhang
Sep 21, 2017·Nature·Janice S ChenJennifer A Doudna
Nov 23, 2017·Nucleic Acids Research·Omri MalikAriel Kaplan
Jan 26, 2018·Angewandte Chemie·Anastasia KadinaEric T Kool
Feb 24, 2018·Journal of the American Chemical Society·Willem A VelemaEric T Kool
Jul 26, 2018·Cell Reports·Akshay TambeMitchell R O'Connell

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Citations

Sep 1, 2020·Journal of the American Chemical Society·Lu XiaoEric T Kool

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Methods Mentioned

BETA
in vitro transcription
electrophoresis
electrophoretic mobility shift assay
nucleic acid cleavage
Circular Dichroism

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