The MAPK kinase BcMkk1 suppresses oxalic acid biosynthesis via impeding phosphorylation of BcRim15 by BcSch9 in Botrytis cinerea

PLoS Pathogens
Yanni YinZhonghua Ma

Abstract

The mitogen-activated protein kinase (MAPK) cassette of the cell wall integrity (CWI) pathway is primarily responsible for orchestrating changes of cell wall. However, functions of this cassette in other cellular processes are not well understood. Here, we found that the Botrytis cinerea mutant of MAPK kinase (BcMkk1) displays more serious defects in mycelial growth, conidiation, responses to cell wall and oxidative stresses, but possesses less reduced virulence than the mutants of its upstream (BcBck1) and downstream (BcBmp3) kinases. Interestingly, BcMkk1, but not BcBck1 and BcBmp3, negatively regulates production of oxalic acid (OA) and activity of extracellular hydrolases (EHs) that are proposed to be virulence factors of B. cinerea. Moreover, we obtained evidence that BcMkk1 negatively controls OA production via impeding phosphorylation of the Per-Arnt-Sim (PAS) kinase BcRim15 by the Ser/Thr kinase BcSch9. In addition, the fungal Pro40 homolog BcPro40 was found to interact simultaneously with three MAPKs, implying that BcPro40 is a scaffold protein of the CWI pathway in B. cinerea. Taken together, results of this study reveal that BcMkk1 negatively modulates virulence via suppressing OA biosynthesis in B. cinerea, which pr...Continue Reading

References

Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·B N Lee, E A Elion
Apr 8, 2000·Molecular Plant-microbe Interactions : MPMI·Y TakanoI Furusawa
Sep 12, 2000·FEMS Microbiology Letters·N L Gil-adA M Mayer
Jan 5, 2002·Molecular Plant-microbe Interactions : MPMI·C S GronoverB Tudzynski
Apr 4, 2002·Molecular Microbiology·Alexander SchoutenJan A L van Kan
Aug 11, 2004·Cell Cycle·Elisabetta CameroniClaudio De Virgilio
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Jae-Hyuk YuClaudio Scazzocchio
Oct 27, 2004·Plant Physiology·Rejane L Guimarães, Henrik U Stotz
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
Dec 13, 2005·Molecular & Cellular Proteomics : MCP·Eiji KinoshitaTohru Koike
Mar 22, 2006·Microbiology·R Alonso MongeJ Pla
Apr 18, 2006·Trends in Plant Science·Jan A L van Kan
Jun 9, 2006·Cell Division·Erwin SwinnenJoris Winderickx
Sep 1, 2006·Molecular Plant-microbe Interactions : MPMI·Muriel ViaudLaurent Legendre
Jan 25, 2007·The Journal of Biological Chemistry·Ying HanDebra Dunaway-Mariano
Jul 3, 2007·Eukaryotic Cell·Tomonori FujiokaKeietsu Abe
Aug 24, 2007·Eukaryotic Cell·Xinhua ZhaoJin-Rong Xu
Oct 27, 2009·Fungal Genetics and Biology : FG & B·Arjen ten HaveJan A L van Kan
Sep 1, 2007·Molecular Plant Pathology·Brian WilliamsonJan A L van Kan
Jun 19, 2010·The Journal of Biological Chemistry·Chen ChenOsnat Herzberg
Sep 25, 2010·Omics : a Journal of Integrative Biology·Luciano GaldieriAles Vancura
Oct 5, 2010·The FEBS Journal·László Buday, Péter Tompa

❮ Previous
Next ❯

Citations

Apr 10, 2021·The Plant Journal : for Cell and Molecular Biology·Tuo YangJi Tian
Aug 29, 2020·Journal of Agricultural and Food Chemistry·Hui XiongQingchun Huang

❮ Previous
Next ❯

Methods Mentioned

BETA
nucleotide exchange
PCR
reverse transcription PCR
transmission electron microscopy
affinity capture assay
two-hybrid
Y2H
pulled down
pull-down
affinity

Software Mentioned

Mega
IMAGE QUANT

Related Concepts

Related Feeds