The MAPK Kinase Kinase GmMEKK1 Regulates Cell Death and Defense Responses

Plant Physiology
Hui-Yang XuJian-Zhong Liu

Abstract

MAPK signaling pathways play critical roles in plant immunity. Here, we silenced multiple genes encoding MAPKs using virus-induced gene silencing mediated by Bean pod mottle virus to identify MAPK genes involved in soybean (Glycine max) immunity. Surprisingly, a strong hypersensitive response (HR) cell death was observed when soybean MAPK KINASE KINASE1 (GmMEKK1), a homolog of Arabidopsis (Arabidopsis thaliana) MEKK1, was silenced. The HR was accompanied by the overaccumulation of defense signaling molecules, salicylic acid (SA) and hydrogen peroxide. Genes involved in primary metabolism, translation/transcription, photosynthesis, and growth/development were down-regulated in GmMEKK1-silenced plants, while the expression of defense-related genes was activated. Accordingly, GmMEKK1-silenced plants were more resistant to downy mildew (Peronospora manshurica) and Soybean mosaic virus compared with control plants. Silencing GmMEKK1 reduced the activation of GmMPK6 but enhanced the activation of GmMPK3 in response to flg22 peptide. Unlike Arabidopsis MPK4, GmMPK4 was not activated by either flg22 or SA. Interestingly, transient overexpression of GmMEKK1 in Nicotiana benthamiana also induced HR. Our results indicate that GmMEKK1 play...Continue Reading

Citations

Mar 21, 2020·Annual Review of Plant Biology·Wei LiGuo-Liang Wang
Jan 24, 2019·Frontiers in Plant Science·Xiquan GaoBaomin Feng
Sep 22, 2020·Plant Biotechnology Journal·Micheline N NgakiMadan K Bhattacharyya
Mar 2, 2021·Frontiers in Plant Science·Zhen-Chao LiJian-Zhong Liu
May 11, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Sumithra Devi ManiRadhakrishnan Nagarathnam
Jan 19, 2022·EMBO Reports·Tongjun Sun, Yuelin Zhang

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