The MAPKKK gene family in cassava: Genome-wide identification and expression analysis against drought stress

Scientific Reports
Jianqiu YeWei Hu

Abstract

Mitogen-activated protein kinase kinase kinases (MAPKKKs), an important unit of MAPK cascade, play crucial roles in plant development and response to various stresses. However, little is known concerning the MAPKKK family in the important subtropical and tropical crop cassava. In this study, 62 MAPKKK genes were identified in the cassava genome, and were classified into 3 subfamilies based on phylogenetic analysis. Most of MAPKKKs in the same subfamily shared similar gene structures and conserved motifs. The comprehensive transcriptome analysis showed that MAPKKK genes participated in tissue development and response to drought stress. Comparative expression profiles revealed that many MAPKKK genes were activated in cultivated varieties SC124 and Arg7 and the function of MeMAPKKKs in drought resistance may be different between SC124/Arg7 and W14. Expression analyses of the 7 selected MeMAPKKK genes showed that most of them were significantly upregulated by osmotic, salt and ABA treatments, whereas slightly induced by H2O2 and cold stresses. Taken together, this study identified candidate MeMAPKKK genes for genetic improvement of abiotic stress resistance and provided new insights into MAPKKK -mediated cassava resistance to droug...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Aug 17, 2002·Current Opinion in Plant Biology·Claudia JonakHeribert Hirt
Jun 26, 2003·Nucleic Acids Research·Elisabeth GasteigerAmos Bairoch
Apr 10, 2004·Journal of Experimental Botany·Huixia ShouKan Wang
May 18, 2004·The Plant Journal : for Cell and Molecular Biology·Yule LiuS P Dinesh-Kumar
Jul 22, 2005·Annals of Botany·Longbin HuangBernard Dell
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Nov 21, 2007·Methods in Molecular Biology·Nicola Mulder, Rolf Apweiler
Jun 17, 2009·Molecular Plant·Andrea PitzschkeHeribert Hirt
Apr 17, 2010·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Kudupudi Prabhakara RaoAlok Krishna Sinha
Jul 6, 2010·Gene·Mohammed NuruzzamanShoshi Kikuchi
May 18, 2011·The Plant Journal : for Cell and Molecular Biology·Qingning ZengBrian E Ellis
May 20, 2011·Nucleic Acids Research·Robert D FinnSean R Eddy
Apr 24, 2012·Tropical Plant Biology·Simon ProchnikSteve Rounsley
Feb 23, 2013·Environmental Science and Pollution Research International·Feng Yun ZhaoTao Liu
May 18, 2013·Bioinformatics·Paul BrownSascha Ott
Jun 12, 2013·Bioinformation·Peng Wan, Dongsheng Che
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta
Sep 2, 2014·Genetics and Molecular Research : GMR·E J OliveiraV S Santos
Oct 29, 2014·Nucleic Acids Research·UNKNOWN UniProt Consortium
Nov 22, 2014·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Dec 17, 2014·Bioinformatics·Bo HuGe Gao
Feb 15, 2015·Plant Molecular Biology·Daisuke MatsuokaTakashi Nanmori
Aug 11, 2015·Frontiers in Plant Science·Birsen Çakır, Ozan Kılıçkaya
May 5, 2016·Trends in Plant Science·Axel de ZelicourtHeribert Hirt
Jan 31, 2017·Biochemical and Biophysical Research Communications·Yuanyuan LiChengchao Zheng

❮ Previous
Next ❯

Citations

May 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chunlai WuGuangyuan He
Apr 17, 2020·International Journal of Molecular Sciences·Ali Anwar, Ju-Kon Kim
Jun 13, 2021·Scientific Reports·Chengzheng YuMadhu Khanna

❮ Previous
Next ❯

Methods Mentioned

BETA
environmental stress
RNA-seq
cDNA library

Software Mentioned

MEME
InterPro Scan
FastQC
Clustal X
MEGA
BLAST
DEGseq
Gene Structure Display Server ( GSDS )
Clustal X2
Tophat

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.