The MapZ-Mediated Methylation of Chemoreceptors Contributes to Pathogenicity of Pseudomonas aeruginosa

Frontiers in Microbiology
Shuo ShengLinghui Xu

Abstract

The pathogenic bacterium Pseudomonas aeruginosa is notorious for causing acute and chronic infections in humans. The ability to infect host by P. aeruginosa is dependent on a complex cellular signaling network, which includes a large number of chemosensory signaling pathways that rely on the methyl-accepting chemotaxis proteins (MCPs). We previously found that the second messenger c-di-GMP-binding adaptor MapZ modulates the methylation of an amino acid-detecting MCP by directly interacting with a chemotaxis methyltransferase CheR1. The current study further expands our understanding of the role of MapZ in regulating chemosensory pathways by demonstrating that MapZ suppresses the methylation of multiple MCPs in P. aeruginosa PAO1. The MCPs under the control of MapZ include five MCPs (Aer, CtpH, CptM, PctA, and PctB) for detecting oxygen/energy, inorganic phosphate, malate and amino acids, and three MCPs (PA1251, PA1608, and PA2867) for detecting unknown chemoattractant or chemorepellent. Chemotaxis assays showed that overexpression of MapZ hampered the taxis of P. aeruginosa toward chemoattractants and scratch-wounded human cells. Mouse infection experiments demonstrated that a dysfunction in MapZ regulation had a profound negat...Continue Reading

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Citations

Apr 25, 2020·Environmental Microbiology·Guang-Lei MaZhao-Xun Liang
Jul 28, 2019·The Journal of Biological Chemistry·Lingyi XinZhao-Xun Liang
Jun 9, 2020·Biotechnology Journal·Zhao LiuChun Loong Ho
Sep 30, 2020·FEMS Microbiology Reviews·Changhan LeeUte Römling
Jan 15, 2021·Microbiology and Molecular Biology Reviews : MMBR·Miguel A MatillaTino Krell

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Datasets Mentioned

BETA
HCB721

Methods Mentioned

BETA
methyltransferase assay
PCR
methylation assay

Software Mentioned

MapZ
Axiovision

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