The master transcription factor Spo0A is required for poly(3-hydroxybutyrate) (PHB) accumulation and expression of genes involved in PHB biosynthesis in Bacillus thuringiensis

FEMS Microbiology Letters
Hui-Ju ChenGwo-Chyuan Shaw

Abstract

Bacillus thuringiensis is a gram-positive spore-forming bacterium that can accumulate poly(3-hydroxybutyrate) (PHB) as a carbon and energy storage substance in response to nutritional stress. The regulatory mechanism for PHB biosynthesis in B. thuringiensis and diverse Bacillus species is still poorly understood. We now report that disruption of the sigH gene or the gene encoding the master sporulation transcription factor Spo0A severely impaired PHB accumulation in B. thuringiensis. Complementation of the spo0A mutation with the spo0A gene restored PHB accumulation. We have found that the requirement of Spo0A for PHB accumulation is independent of the transition state regulator AbrB and of loss of sporulation ability. We also show that Spo0A is required for the expression of three genes involved in PHB biosynthesis. These findings have uncovered a new role of Spo0A in the regulation of stationary-phase-associated cellular events.

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M StrauchJ A Hoch
Nov 1, 1990·Molecular Microbiology·P Stragier, R Losick
Nov 1, 1965·Journal of Bacteriology·L A Kominek, H O Halvorson
Dec 29, 1995·Gene·A M Guérout-FleuryP Stragier
Sep 25, 2002·Journal of Bacteriology·Sinda FedhilaDidier Lereclus
Jun 21, 2003·Science·José E González-PastorRichard Losick
Jul 1, 1961·Journal of Bacteriology·J H LAW, R A SLEPECKY
Dec 4, 2003·Molecular Microbiology·Virginie MolleRichard Losick
Nov 6, 2004·Applied and Environmental Microbiology·Maryvonne ArnaudMichel Débarbouillé
Nov 24, 2004·Current Opinion in Microbiology·Patrick J Piggot, David W Hilbert
Feb 3, 2005·Journal of Bacteriology·Masaya FujitaRichard Losick
Feb 15, 2007·Journal of Bioscience and Bioengineering·Takeko KodamaJunichi Sekiguchi
Mar 10, 2009·Journal of Bacteriology·Dieter Jendrossek

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