The MATK tyrosine kinase interacts in a specific and SH2-dependent manner with c-Kit.

The Journal of Biological Chemistry
B H JhunH Avraham

Abstract

We have cloned a protein tyrosine kinase, MATK, which is expressed abundantly in megakaryocytes and the brain. We investigated whether MATK participates in the c-Kit ligand/stem cell factor (KL/SCF) signaling pathway in the megakaryocytic cell line CMK. After KL/SCF stimulation, five major proteins of molecular masses of 145, 113, 92, 76, and 63 kDa were rapidly and transiently tyrosine-phosphorylated in a time-dependent manner, peaking within 5 min, and returning to basal levels within 60 min. To study the role of MATK in the KL/SCF signaling pathway, glutathione S-transferase (GST) fusion proteins containing SH2 and SH3 domains of MATK were cloned, expressed in Escherichia coli, and purified. MATK-SH2, but not MATK-SH3, precipitated the tyrosine-phosphorylated c-Kit (molecular mass of 145 kDa) in KL/SCF-stimulated CMK cells. Other GST fusion proteins containing the SH2 domain of p85 of phosphatidylinositol 3-kinase, phospholipase C gamma-1, and ras-GAP also precipitated c-Kit. The tyrosine-phosphorylated c-Kit was co-immunoprecipitated with anti-MATK and anti-p85 antibodies in KL/SCF-stimulated CMK cells, but not in granulocyte-macrophage colony stimulating factor or interleukin-6-stimulated cells, suggesting receptor specifi...Continue Reading

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Citations

Jul 11, 1998·Biochimica Et Biophysica Acta·M A FarrarK Sauer
Dec 3, 1999·The International Journal of Biochemistry & Cell Biology·D Linnekin
Jul 1, 1995·Stem Cells·H AvrahamS Avraham
Feb 15, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jung Ha KimNacksung Kim
Jan 17, 1997·The Journal of Biological Chemistry·S Zrihan-LichtH Avraham
Jul 11, 2001·The Journal of Biological Chemistry·C BougeretH Avraham
Apr 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David KentConnie Eaves

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