The maturation state of three types of granulocyte/macrophage progenitor cells from mouse bone marrow

Journal of Cellular Physiology
S Bol, N Williams

Abstract

The progenitor cells of neutrophil granulocytes and macrophages which are able to proliferate and differentiate in vitro (CFU-c) form a heterogeneous population. By the use of specific colony stimulating activities and cell separation by equilibrium density centrifugation, three subpopulations of CFU-c can be detected. These three CFU-c are characterized by buoyant densities of 1.070, 1.075 and 1.080 g.cm-3 and by their proliferative response to 18 h postendotoxin serum, colony stimulating factor from extracts of mouse embryos and uteri (CSF-pmue) and erythrocyte lysate, respectively. The three CFU-c are compared with respect to their differentiation potential, the maturation rate of their progeny cells and their proliferation capacity. It is shown that with increasing density of the CFU-c the maturation rate increases (sequential maturation of colonies derived from CFU-c with densities of 1.080, 1.075, 1.070 g.cm-3) and the proliferation capacity decreases (colony size decreases in the sequence of CFU-c with densities 1.070, 1.075, 1.080 g.cm-3). Concerning the differentiation potential it is shown that all three CFU-c detected have the capacity to form granulocytes as well as macrophages. On the basis of these results it is c...Continue Reading

References

Nov 1, 1977·Experimental Cell Research·M Horiuchi, Y Ichikawa
Jan 1, 1978·Cell and Tissue Kinetics·G Wagemaker, M F Peters
Nov 1, 1975·Cell and Tissue Kinetics·G van den Engh, S Bol
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Dec 1, 1971·The Australian Journal of Experimental Biology and Medical Science·T R BradleyM A Sumner

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Citations

Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H M GarnettK Harigaya
Jun 1, 1984·The Journal of Experimental Medicine·J W VisserA M de Leeuw
Oct 1, 1984·Journal of Immunogenetics·R A HarrisI F McKenzie
Mar 1, 1984·Cell and Tissue Kinetics·S AizawaK J Mori
Jan 1, 1984·Critical Reviews in Oncology/hematology·H E Broxmeyer
Jun 1, 1982·Journal of Cellular Physiology·D Metcalf, A W Burgess
May 1, 1980·Journal of Cellular Physiology·N Williams, H Jackson
Jan 1, 1988·Critical Reviews in Oncology/hematology·H E Broxmeyer, D E Williams

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