The maximal downstroke of epicardial potentials as an index of electrical activity in mouse hearts

IEEE Transactions on Bio-medical Engineering
Kwanghyun SohnB B Punske

Abstract

The maximal upstroke of transmembrane voltage (dV(m)/dt(max)) has been used as an indirect measure of sodium current I(Na) upon activation in cardiac myocytes. However, sodium influx generates not only the upstroke of V(m), but also the downstroke of the extracellular potentials V(e) including epicardial surface potentials V(es). The purpose of this study was to evaluate the magnitude of the maximal downstroke of V(es) (|dV(es)/dt (min)|) as a global index of electrical activation, based on the relationship of dV(m)/dt(max) to I(Na). To fulfill this purpose, we examined |dV(es)/dt(min)| experimentally using isolated perfused mouse hearts and computationally using a 3-D cardiac tissue bidomain model. In experimental studies, a custom-made cylindrical "cage" array with 64 electrodes was slipped over mouse hearts to measure V(es) during hyperkalemia, ischemia, and hypoxia, which are conditions that decrease I(Na). Values of |dV(es)/dt(min)| from each electrode were normalized (|dV(es)/dt (min)|(n)) and averaged (|dV(es)/dt(min)|(na)). Results showed that |dV(es)/dt(min)|(na) decreased during hyperkalemia by 28, 59, and 79% at 8, 10, and 12 mM [K(+)](o), respectively. |dV(es)/dt(min)| also decreased by 54 and 84% 20 min after the o...Continue Reading

References

Jul 1, 1978·Biophysical Journal·G Strichartz, I Cohen
Aug 1, 1992·Circulation Research·G X Yan, A G Kléber
May 1, 1991·Circulation Research·E MurphyC Steenbergen
Aug 1, 1988·Circulation Research·M F SheetsH A Fozzard
Jul 1, 1987·IEEE Transactions on Bio-medical Engineering·S M BlanchardJ E Lowe
Aug 1, 1987·Computers and Biomedical Research, an International Journal·F A Roberge, J P Drouhard
Jul 1, 1985·IEEE Transactions on Bio-medical Engineering·F J ClaydonR E Ideker
Aug 1, 1974·The Journal of Physiology·L S Gettes, H Reuter
Dec 9, 1981·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·I CohenG Strichartz
Feb 1, 1984·Pflügers Archiv : European journal of physiology·G A Gintant, B F Hoffman
Jan 1, 1996·Journal of Molecular and Cellular Cardiology·J T VermeulenM J Janse
Feb 5, 2003·Canadian Journal of Physiology and Pharmacology·Cindy TissierPierre Athias
Sep 16, 2003·Annals of Biomedical Engineering·Bonnie B PunskeBruno Taccardi
Sep 1, 1951·The Journal of Physiology·M H DRAPER, S WEIDMANN
Oct 18, 2005·Journal of Electrocardiology·Bonnie B PunskeSheldon E Litwin
Oct 20, 2007·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Qiansheng LiangBonnie B Punske
Mar 3, 1949·The Journal of Physiology·A L HODGKIN, B KATZ
Feb 12, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Bruno TaccardiPhilip R Ershler
Mar 14, 2009·Annals of Biomedical Engineering·Frank B SachseJ A Abildskov

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Citations

Nov 28, 2012·Experimental Physiology·Kwanghyun SohnBonnie B Punske

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