The Mcm2-Ctf4-Polα Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands.

Molecular Cell
Haiyun GanZhiguo Zhang

Abstract

Although essential for epigenetic inheritance, the transfer of parental histone (H3-H4)2 tetramers that contain epigenetic modifications to replicating DNA strands is poorly understood. Here, we show that the Mcm2-Ctf4-Polα axis facilitates the transfer of parental (H3-H4)2 tetramers to lagging-strand DNA at replication forks. Mutating the conserved histone-binding domain of the Mcm2 subunit of the CMG (Cdc45-MCM-GINS) DNA helicase, which translocates along the leading-strand template, results in a marked enrichment of parental (H3-H4)2 on leading strand, due to the impairment of the transfer of parental (H3-H4)2 to lagging strands. Similar effects are observed in Ctf4 and Polα primase mutants that disrupt the connection of the CMG helicase to Polα that resides on lagging-strand template. Our results support a model whereby parental (H3-H4)2 complexes displaced from nucleosomes by DNA unwinding at replication forks are transferred by the CMG-Ctf4-Polα complex to lagging-strand DNA for nucleosome assembly at the original location.

Citations

Oct 16, 2019·ELife·Daniel S Saxton, Jasper Rine
Oct 3, 2019·Annual Review of Genetics·Stanislau YatskevichKim Nasmyth
May 6, 2020·Biochemical Society Transactions·Jacob S Lewis, Alessandro Costa
Apr 25, 2019·Essays in Biochemistry·Alonso J PardalAndrew J Bowman
Sep 9, 2020·Nature Structural & Molecular Biology·Júlia TornéGeneviève Almouzni
Apr 2, 2020·Epigenetics : Official Journal of the DNA Methylation Society·Thom M MolenaarFred van Leeuwen
Apr 2, 2020·Nature Cell Biology·Kathleen R Stewart-MorganAnja Groth
Aug 10, 2019·Biochemical Society Transactions·Nitin Kapadia, Rodrigo Reyes-Lamothe
Mar 27, 2020·Frontiers in Plant Science·Alice HuguesFrançois Roudier
Aug 28, 2020·Frontiers in Cell and Developmental Biology·William A Scott, Eric I Campos
Mar 20, 2020·Cell & Bioscience·Wenshuo ZhangQing Li
Oct 27, 2020·Nucleic Acids Research·Tim Formosa, Fred Winston
Nov 10, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Lucia Falbo, Vincenzo Costanzo
Sep 12, 2020·The Journal of Biological Chemistry·Melanie A SparksRoberto Galletto
Dec 11, 2020·Nucleic Acids Research·Nataliya PetrykPierre-Antoine Defossez
Jan 28, 2021·Nature Reviews. Genetics·Thelma M EscobarDanny Reinberg
May 6, 2020·Molecular Cell·Domagoj BaretićJoseph T P Yeeles
Mar 23, 2021·Frontiers in Genetics·Kholoud ShabanKrassimir Yankulov
Mar 24, 2021·Molecular and Cellular Biology·Ann K Hogan, Daniel R Foltz
May 21, 2021·PloS One·Mark BoltengagenAnn E Ehrenhofer-Murray
Jun 5, 2021·Mutation Research. Reviews in Mutation Research·Maddalena MognatoKai Rothkamm
Jun 5, 2021·DNA Repair·Ian Hammond-MartelHugo Wurtele
Mar 31, 2021·Annual Review of Biochemistry·Ilan AttaliJames M Berger
Sep 3, 2021·Nature Communications·Erika ChacinChristoph F Kurat
Nov 3, 2021·The FEBS Journal·Chun-Min ShanSongtao Jia
Nov 18, 2021·The EMBO Journal·Ann K HoganDaniel R Foltz

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