The MCP-1/CCR2 system has direct proinflammatory effects in human mesangial cells

Kidney International
S GiuntiGabriella Gruden

Abstract

Both inflammatory and haemodynamic factors have been implicated in the pathogenesis of diabetic and other progressive glomerulopathies. Mesangial cell exposure to mechanical stretch induces both intercellular adhesion molecule 1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1) expression. CC Chemokine receptor 2 (CCR2), the cognate MCP-1 receptor, has been recently demonstrated in human mesangial cells (HMCs). We tested whether MCP-1 binding to CCR2 affects ICAM-1 expression in HMCs and, secondly, if stretch-induced ICAM-1 is mediated by MCP-1 via an autocrine mechanism. Serum-deprived HMCs were exposed to either rh-MCP-1 (0.1-1-10-50-100 ng/ml) or mechanical stretch in the presence and in the absence of RS102895, a specific CCR2 inhibitor. ICAM-1 expression was assessed both by immunofluorescence and cytofluorimetry. Monocyte-HMC interaction was tested by adhesion assay. CCR2 expression was studied by reverse transcriptase-polymerase chain reaction, immunoblotting, and flow cytometry. HMCs exposure to rh-MCP-1 induced a significant twofold increase in ICAM-1 expression at 24 h, leading to enhanced monocyte adhesion. This effect occurred via the CCR2 receptor as CCR2 was expressed in HMCs and CCR2 blockade prevented ICAM...Continue Reading

References

Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·H BeaufilsG Chomette
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·I F CharoS R Coughlin
Sep 30, 1993·The New England Journal of Medicine·UNKNOWN Diabetes Control and Complications Trial Research GroupC Siebert
May 1, 1993·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·T FurutaK Yoshinaga
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·G GrudenG Viberti
Sep 1, 1999·Kidney International·S KatoH S MacKenzie
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A SicaA Mantovani
Apr 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Robert E Gerszten
Apr 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Merce RoqueMark B Taubman
May 3, 2002·Clinical and Experimental Immunology·M SchwarzH H Radeke
Dec 11, 2002·Kidney International·Ricardo UtimuraIrene De Lourdes Noronha
May 16, 2003·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jaimar RinconJesus A Mosquera
May 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Peter Sartipy, David J Loskutoff
Nov 2, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ulrich A MausJürgen Lohmeyer

❮ Previous
Next ❯

Citations

Oct 19, 2011·Apoptosis : an International Journal on Programmed Cell Death·Bo Young NamShin-Wook Kang
Jan 14, 2011·Hypertension Research : Official Journal of the Japanese Society of Hypertension·José Butori Lopes de FariaJacqueline Mendonça Lopes de Faria
Sep 3, 2011·Critical Care : the Official Journal of the Critical Care Forum·Jan Willem KuiperA B Johan Groeneveld
Nov 20, 2012·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Heloisa Reniers ViannaAna Cristina Simões E Silva
Jun 1, 2010·Translational Stroke Research·Aaron A Hall, Keith R Pennypacker
Jan 8, 2016·Human Molecular Genetics·Waylan K BesslerBrian K Stansfield
Jul 17, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Amita YadavSarika Arora
Dec 2, 2008·Clinical and Experimental Immunology·L M McIntoshJ R McDonald
Apr 3, 2014·Disease Markers·Ana Cristina Simões e SilvaAntônio Lúcio Teixeira
Aug 20, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Min Jeong Kim, Frederick W K Tam
Sep 6, 2006·Hypertension·Sara GiuntiMark E Cooper
Apr 19, 2019·Diabetes/metabolism Research and Reviews·Federica BaruttaGabriella Gruden
Dec 3, 2015·Current Opinion in Nephrology and Hypertension·Hermann HallerJan Menne
Jun 27, 2008·American Journal of Physiology. Renal Physiology·Jehyun ParkShin-Wook Kang
Feb 14, 2014·American Journal of Physiology. Renal Physiology·Qiongzhen LinLi Yang
Mar 30, 2020·Journal of Nephrology·Federica BaruttaGabriella Gruden
Oct 17, 2008·American Journal of Physiology. Renal Physiology·Federica BaruttaGabriella Gruden
Feb 1, 2013·American Journal of Physiology. Renal Physiology·Diping WangJoseph P Grande
Feb 8, 2020·International Journal of Molecular Sciences·Tian-Yu Lin, Yu-Hsiang Hsu
Apr 8, 2017·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Federica BaruttaGabriella Gruden
Feb 12, 2021·Frontiers in Cell and Developmental Biology·Hong ZhouLi-Qin Tang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.