PMID: 9444942Jan 28, 1998Paper

The MDR1 (P-glycoprotein) and MRP (P-190) transporters do not play a major role in the intrinsic multiple drug resistance of Jurkat T lymphocytes

Leukemia Research
J MartelG Dupuis

Abstract

The response of T cells in relation to the cell cycle has not been extensively studied. We have attempted to address this question using Jurkat T cells treated with cytostatic drugs known to arrest cells at various transition points of their cycle. We tested several concentrations of drugs that act at G1/S (hydroxyurea, lovastatin, thymidine), early S (aphidicolin, cyclosporin A, rapamycin) or G2+M (colchicine, nocodazole) in 24 h cultures. Cytofluorimetric analyses showed that cycling Jurkat cells were equally distributed between the G1 (44.9 +/- 6.5%) and S (42.3 +/- 8.0%) phases. Cell distribution in G2+M was 12.7 +/- 2.8%. Hydroxyurea but not lovastatin increased the percentage of cells in S phase to approximately 60-70% and both drugs decreased it to approximately 30% in G1. Thymidine had no effects. Aphidicolin increased the distribution in S phase to approximately 70% with a decrease in G1 to approximately 30%. Cyclosporin A and rapamycin increased the percentage of the cells in G1 to approximately 70% and decreased it to approximately 25% in S phase. Nocodazole increased cell distribution in G2+M to approximately 60% and induced a decrease in G1 to approximately 10%. The effects of the drugs were not related to their to...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·L Thelander, P Reichard
Jun 1, 1976·Scandinavian Journal of Immunology·A Bøyum
Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Mar 1, 1992·Canadian Journal of Physiology and Pharmacology·S P Cole
Jul 7, 1992·Biochimica Et Biophysica Acta·D Nielsen, T Skovsgaard
Apr 1, 1992·Immunology Today·S L Schreiber, G R Crabtree
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M JakóbisiakZ Darzynkiewicz
Jun 30, 1987·Biochemical and Biophysical Research Communications·T McGrath, M S Center
Jan 1, 1986·Annual Review of Immunology·A WeissJ Stobo
Jan 1, 1988·Experimental Cell Research·B Maurer-SchultzeI D Bassukas
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K UedaI Pastan
Mar 1, 1985·European Journal of Immunology·U LandegrenH Wigzell
Jun 1, 1973·The Journal of Cell Biology·K PorterJ Frye
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A W AlbertsJ Springer
Jan 1, 1982·Leukemia Research·P C VincentR A Zbroja
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·G J ZamanP Borst
Apr 15, 1993·Biochemical and Biophysical Research Communications·K NoguchiT Tsuruo
Jan 1, 1995·The Journal of Membrane Biology·C R Leveille-Webster, I M Arias
Jan 1, 1994·Annual Review of Biochemistry·G R Crabtree, N A Clipstone
Mar 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M Clynes
Jan 1, 1993·Annual Review of Biochemistry·M M Gottesman, I Pastan
Sep 30, 1993·International Journal of Cancer. Journal International Du Cancer·A H SchinkelP Borst
Jan 28, 1994·Cell·A Weiss, D R Littman
Jan 1, 1993·Annual Review of Immunology·R M PerlmutterJ Alberola-Ila
Apr 1, 1993·Current Biology : CB·E Karsenti
Dec 12, 1986·Science·E Lazarides

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Related Papers

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
H van de Waterbeemd
Biochemistry and Molecular Biology International
A SokalG Bartosz
Proceedings of the National Academy of Sciences of the United States of America
Sungpil ChoWilliam T Beck
© 2021 Meta ULC. All rights reserved