PMID: 1197784Sep 1, 1975Paper

The measurement of E and 19-hydroxy E prostaglandins in human seminal plasma

Prostaglandins
I Cooper, R W Kelly

Abstract

A method is described which measures the four main prostaglandins of human semen (PGE1, E2, 19-hydroxy PGE1, and 19-hydroxy PGE2). For routine measurements E1 and E2 are measured together as are 19-OH E1 and 19-OH E2. These are measured by forming oximes in aqueous solution extraction, methylation and trimethyl silylation followed by gas chromatography. The method has sufficient sensitivity to measure the levels found in the majority of semen samples. The normal range in men with proven fertility was 90 to 260 mug/ml of 19-hydroxy Es and 30-200 mug/ml of Es.

References

Aug 1, 1970·Fertility and Sterility·M BygdemanB Samuelsson

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Citations

Apr 1, 1977·Prostaglandins·C H SpilmanA D Forbes
Feb 1, 1982·Theriogenology·W BielańskiA Okólski
Jan 1, 1981·Progress in Lipid Research·H T Jonsson, R E Powers
Jan 1, 1979·Preparative Biochemistry·P L Taylor, R W Kelly
Sep 1, 1975·FEBS Letters·P L Taylor, R W Kelly
Jan 1, 1988·Prostaglandins, Leukotrienes, and Essential Fatty Acids·R FreixiaE Gelpi
Apr 1, 1987·International Journal of Andrology·E BendvoldP Eneroth

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