PMID: 9438066Jan 23, 1998Paper

The measurement of lipid peroxidation in vivo

Brain Research. Brain Research Protocols
A H WaterfallC A Marsden

Abstract

Tissue damage due to free radical activity may be a common mediator in pathology [Kehrer, Crit. Rev. Toxicol. 23 (1993) 21-48]. The confirmation that free radicals have a role in the disease process is hampered by the difficulty of their measurement, as free radicals are transient species present at low concentrations in tissue. Direct measurement of free radicals is confined to electron spin resonance, a specialised methodology not available in our laboratory. We have evaluated several indirect measures, namely, changes in antioxidant status, hydroxyl radical trapping assays and monitoring breakdown products of free radical attack. Here we describe a method from the latter category, monitoring lipid peroxidation. The measurement of lipid peroxidation has been favoured as the indicator of free radical activity in previous ex vivo and in vitro studies of oxidative stress, in particular by the measurement of malondialdehyde (MDA). There is an absence of reported measurements of MDA from in vivo preparations especially where samples are collected in situ. The method describes the combination of microdialysis sampling with high performance liquid chromatography coupled to ultraviolet spectroscopy, to measure MDA from in vivo prepar...Continue Reading

Citations

Jun 11, 2009·Bulletin of Experimental Biology and Medicine·I D SafronovE D Kokareva
Dec 29, 2012·Neurotoxicology·Monique ChalansonnetFrançois Gagnaire
Jul 23, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Shuang GaoBing Li
Nov 30, 2018·Naunyn-Schmiedeberg's Archives of Pharmacology·Priya J GhumatkarSadhana Sathaye

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