PMID: 15385840Sep 24, 2004Paper

The measurement of nicotine in human plasma by high-performance liquid chromatography-electrospray-tandem mass spectrometry

Therapeutic Drug Monitoring
P J TaylorPeter I Pillans

Abstract

The authors describe a reverse-phase high-performance liquid chromatography-electrospray-tandem mass spectrometry method for the measurement of nicotine in human plasma. Samples (500 microL) with added deuterium-labeled d3-nicotine as an internal standard (IS) were treated with a 2-step process of ether extraction (6 mL) followed by back-extraction into 0.1% formic acid (50 microL). Chromatography was performed on a phenyl Novapak column with a mobile phase consisting of 50% 10 mM ammonium formate (pH 3.3) and acetonitrile (50:50, vol/vol). A flow rate of 0.2 mL/min resulted in a total analysis time of 5 minutes per sample. Mass spectrometric detection was by selected reactant monitoring (nicotine m/z 163.2 --> 130.2; IS m/z 166.2 --> 87.2). The assay was linear from 0.5 to 100 microg/L (r > 0.993, n = 9). The accuracy and imprecision of the method for quality control samples were 87.5% to 113% and <10.2%, respectively. Interday accuracy and imprecision at the limit of quantification (0.5 microg/L) was 113% and 7.2% (n = 4). The process efficiency for nicotine in plasma was >75%. The method described has good process efficiency, stabilized nicotine, avoided concentration steps, and most importantly minimized potential contamina...Continue Reading

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Citations

May 14, 2005·Analytical and Bioanalytical Chemistry·P MassarotiJ Pedrazzoli
Jun 22, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M J BockA Dudley
Mar 8, 2005·Journal of Mass Spectrometry : JMS
Jun 20, 2020·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Osama A AbulseoudElliot Stein
Jul 7, 2005·Phytochemical Analysis : PCA
Sep 28, 2006·Journal of Agricultural and Food Chemistry·Joseph E JablonskiPurnima Mariappagoudar

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