The mechanical properties of chick (Gallus domesticus) sensory hair bundles: relative contributions of structures sensitive to calcium chelation and subtilisin treatment

The Journal of Physiology
Mikhail E BashtanovIan J Russell

Abstract

Up to four link types are found between the stereocilia of chick vestibular hair bundles: tip links, horizontal top connectors, shaft connectors and ankle links. A fifth type, the kinocilial link, couples the hair bundle to the kinocilium. Brownian-motion microinterferometry was used to study the mechanical properties of the hair bundle and investigate changes caused by removing different links with the calcium chelator BAPTA or the protease subtilisin. Immunofluorescence with an antibody to the hair-cell antigen (HCA) and electron microscopy were used to verify destruction of the links. The root mean square displacement and the corresponding absolute stiffness of untreated hair bundles were 4.3 nm and 0.9 mN m(-1), respectively. The ratio of Brownian-motion spectra before and after treatment was calculated and processed using a single oscillator model to obtain relative stiffness. Treatment with BAPTA, which cleaves tip, kinocilial and ankle links, reduces hair-bundle stiffness by 43%, whilst subtilisin treatment, which breaks ankle links and shaft connectors, reduces stiffness by 48%. No changes were detected in viscous damping following either treatment. The time course of the subtilisin-induced stiffness change was close to...Continue Reading

References

Dec 22, 1992·Proceedings. Biological Sciences·I J RussellG P Richardson
May 1, 1992·Hearing Research·Y M SzymkoJ C Saunders
Mar 1, 1991·The Journal of Physiology·A C CrawfordR Fettiplace
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W DenkA J Hudspeth
Nov 1, 1989·The Journal of the Acoustical Society of America·J C Saunders, Y M Szymko
Jan 1, 1986·Hearing Research·J Howard, J F Ashmore
Jul 1, 1985·The Journal of Physiology·A C Crawford, R Fettiplace
Jul 1, 1984·Hearing Research·D Strelioff, A Flock
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S S Pae, J C Saunders
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·R E Marquis, A J Hudspeth
May 24, 2001·Biophysical Journal·M G LangerJ P Ruppersberg
Dec 18, 2001·Hearing Research·J MeyerA W Gummer
Dec 24, 2002·Biophysical Journal·Jonathan E FrankFernán Jaramillo

❮ Previous
Next ❯

Citations

Feb 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrei S KozlovA J Hudspeth
Jul 9, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·K Domenica Karavitaki, David P Corey
Jun 15, 2006·The Journal of Membrane Biology·A J RicciS M Van Netten
Jul 14, 2010·The Journal of Cell Biology·Martin SchwanderUlrich Müller
Aug 15, 2009·Journal of Biomechanics·Koeun Lim, Sukyung Park
Apr 10, 2007·Journal of Communication Disorders·James C Saunders
May 19, 2007·Hearing Research·Peter M Sellick
Jan 8, 2008·Biophysical Journal·Maryline BeurgRobert Fettiplace
Jul 26, 2015·Progress in Biophysics and Molecular Biology·Miljko V SataricSlobodan Zdravkovic
Apr 7, 2005·Hearing Research·Jens MeyerAnthony W Gummer
Oct 7, 2009·Cell·Peter G Gillespie, Ulrich Müller
Sep 20, 2005·Biophysical Journal·Dylan K Chan, A J Hudspeth
Jun 25, 2014·Pflügers Archiv : European journal of physiology·Nicolas Michalski, Christine Petit
Jan 14, 2017·Frontiers in Pediatrics·Tanbir Najrana, Juan Sanchez-Esteban
Jul 25, 2015·PloS One·Gilad OrlyNir S Gov
Nov 21, 2007·Biotechnology and Bioengineering·N B E SawyerS P Morgan
Apr 18, 2019·Journal of Neurophysiology·Jong-Hoon NamE H Peterson
May 4, 2013·Journal of Cell Science·Carole M Hackney, David N Furness

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved