PMID: 20646409Jul 22, 2010Paper

The mechanism and pulmonary-protective effects of endothelin-1 receptor antagonist in chronic obstructive pulmonary diseases rat model

Zhonghua nei ke za zhi
Yan ChenKubo Keishi

Abstract

To investigate whether the endothelin (ET) receptor antagonists have protective role in the development of emphysema. Sprague-Dawley rats (n = 24) were divided into four groups: control group, cigarette smoke extract (CSE) group, BQ123 group and Bosentan group. CSE was injected intraperitoneally once a week for three weeks and BQ123 and Bosentan were administered daily for the same duration. TdT-mediated dUTP nick end labeling (TUNEL) was performed to observe the deoxyribonucleic acid (DNA) damaged cells and the expression of caspase-3 was determined by immunohistochemistry and Western blot. Matrix metalloproteinase-2 (MMP-2) and MMP-9 activities were investigated by gelatin zymography and tumor necrosis factor α (TNFα) and interleukin-1ß (IL-1ß) concentrations were measured by enzyme linked immunosorbent assay (ELISA). We confirmed the emphysematous destruction in the lungs of experimental rats induced by the intraperitoneal injection of CSE within 3 weeks. The mean lining interval (MLI) and damage index (DI) were significantly increased in the CSE group compared with control group. However, the MLI and DI were significantly decreased in the BQ123 and bosentan groups compared with CSE rats (P < 0.01, respectively). The TUNEL-p...Continue Reading

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