The mechanism of action of alpha-amylase from Lactobacillus fermentum on maltooligosaccharides

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
P TalamondAlexandre de Kochko

Abstract

The action pattern of Lactobacillus fermentum alpha-amylase (FERMENTA) was examined using a series of maltooligosaccharides (G2-G7) as substrates. Structurally, this enzyme has a molecular mass (106 kDa) almost twofold higher than alpha-amylases from mammalians and cereals. The product pattern was investigated through an analysis of products and substrates using HPAEC with pulsed amperometric detection. FERMENTA was consistent with an endo-type of amylase. The bond cleavage frequencies were studied using maltooligosaccharides of various chain lengths as substrate, i.e. maltose up to maltoheptaose and DP 4900-amylose catalyzed by FERMENTA. The catalytic efficiency (k(cat)/K(m)) increased with chain length from maltose (8.7 x 10(4) M(-1) s(-1)) up to amylose (1 x10(9) M(-1) s(-1)). These action pattern results revealed that FERMENTA can readily cleave the third linkage from the reducing end of the maltooligosaccharides (G5-G7).

References

Apr 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·J J MarshallW J Whelan
Dec 1, 1965·Analytical Biochemistry·S DygertJ A Thoma
Jan 1, 1983·Molecular and Cellular Biochemistry·K HiromiA Tanaka
Jul 18, 1984·Biochemical and Biophysical Research Communications·E ProdanovG Marchis-Mouren
Sep 15, 1995·Structure·G Davies, B Henrissat
Feb 12, 1998·Protein Science : a Publication of the Protein Society·M QianF Payan
Nov 15, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P TalamondG Marchis-Mouren
Sep 27, 2003·European Journal of Biochemistry·Naïma OudjeriouatVéronique Desseaux

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Citations

Feb 27, 2016·Journal of Agricultural and Food Chemistry·Hye-Yeon JeonJae-Hoon Shim

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