PMID: 6164328Jan 1, 1980Paper

The mechanism of antigenic drift in influenza viruses: analysis of Hong Kong (H3N2) variants with monoclonal antibodies to the hemagglutinin molecule

Annals of the New York Academy of Sciences
R G WebsterK L van Wyke

Abstract

Monoclonal antibodies to the hemagglutinin molecule of the Hong Kong variant, Mem/1/71 (H3N2), influenza virus were used to study antigenic drift in the H3N2 subtype of influenza viruses. Antigenic variants of Mem/1/71 (H3N2) were selected after a single passage of the virus in chick embryos in the presence of monoclonal antibody. The variants showed a marked reduction in the ability to react with the monoclonal antibody used in selection. The monoclonal antibodies could be divided into three groups based on their reactions with the variants, providing evidence for at least three nonoverlapping antigenic areas on the hemagglutinin molecule. Amino acid analysis of tryptic peptides of the hemagglutinin from these variants showed that a single amino acid substitution in the heavy polypeptide chain (HA1) of the hemagglutinin molecule accounted for the reduced antibody interactions, and that variants from each group exhibited sequence changes in different areas of the molecule. Sequence changes were also detected in the HA1 polypeptides of naturally occurring H3N2 variants, but in most cases the changes in the monoclonal antibody selected variants were different from the field strains. Antigenic analysis showed that most of the vari...Continue Reading

References

Aug 1, 1978·Immunochemistry·M Z Atassi, J A Smith
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H KoprowskiC M Croce
Jan 1, 1979·British Medical Bulletin·C W Ward, T A Dopheide
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Feb 25, 1980·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·W G LaverT A Dopheide

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Citations

Jan 1, 1988·Veterinary Research Communications·D E Stallknecht, S M Shane
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R M Iorio, M A Bratt
Mar 30, 2012·Journal of Virology·Jens C KrauseJames E Crowe
Apr 20, 2011·Virology Journal·Rajagowthamee R ThangavelStephen J Stray
Dec 1, 1981·In Vitro·R H Kennett
Mar 7, 2021·International Journal of Environmental Research and Public Health·Rodney P Jones
Mar 19, 1982·Science·P Palese, J F Young

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