PMID: 6402361Jan 1, 1983Paper

The mechanism of binding of L-serine to tryptophan synthase from Escherichia coli

European Journal of Biochemistry
A N Lane, K Kirschner

Abstract

The mechanism of binding of L-serine to tryptophan synthase, which is the initial phase of the catalytic mechanism, has been studied by steady-state and stopped-flow kinetic techniques. The dependence of three separable rate processes on the concentration of L-serine is compatible with four different enzyme-substrate complexes, one of which lies on a branch in the pathway. By use of L-serine deuterated at the alpha carbon, it is possible to assign the deprotonation of the external aldimine of L-serine with pyridoxal 5'-phosphate to the most rapid observable binding step. Measurements at two pH values show that the rate-determining step in the synthesis of L-tryptophan changes from release of L-tryptophan at the optimal pH of 7.6 to the binding of L-serine at pH 6.5. Measurements at pH 7.6 in the presence of the substrate analogue indolepropanol phosphate show that the stronger binding of L-serine is probably due to stabilization of the catalytically competent enzyme--L-serine complex. At pH 7.6 L-serine is bound far more slowly to the beta 2 subunit than to the alpha 2 beta 2 complex of tryptophan synthase and retains its alpha carbon proton. For the beta 2 subunit, the rate-determining step of tryptophan synthesis is deprotona...Continue Reading

References

Dec 1, 1981·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H D Heilmann, M Bürger
Jan 1, 1981·Annual Review of Biochemistry·D B Northrop
Nov 1, 1981·European Journal of Biochemistry·A N Lane, K Kirschner

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Citations

Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·R Bentley
Apr 24, 2009·Cellular and Molecular Life Sciences : CMLS·Samanta RaboniAndrea Mozzarelli
Jan 5, 2002·The Journal of Biological Chemistry·Michael WeyandAndrea Mozzarelli
Nov 11, 2015·Proceedings of the National Academy of Sciences of the United States of America·Andrew R BullerFrances H Arnold
Dec 5, 1998·The Journal of Biological Chemistry·K D Schnackerz, A Mozzarelli
Jan 5, 2002·The Journal of Biological Chemistry·Michael WeyandAndrea Mozzarelli
Jan 1, 1987·Progress in Biophysics and Molecular Biology·R Jaenicke
May 1, 2004·The Journal of Biological Chemistry·Francesca SchiarettiAndrea Mozzarelli
Dec 15, 1995·The Journal of Biological Chemistry·K S AndersonE W Miles
Oct 24, 2009·Biochemistry·Adam T DierkersMichael F Dunn
Dec 20, 1985·Biochimica Et Biophysica Acta·P D TurnerC J Bailey
Nov 1, 1985·Archives of Biochemistry and Biophysics·H Wiesinger, H J Hinz
Oct 1, 1984·Archives of Biochemistry and Biophysics·M L Napier, J K Hardman
Dec 19, 2020·ACS Chemical Biology·Prasanth KumarAndrew R Buller
May 2, 2018·Journal of the American Chemical Society·Andrew R BullerFrances H Arnold
May 20, 2008·Trends in Biochemical Sciences·Michael F DunnIlme Schlichting

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