PMID: 2105747Jan 29, 1990Paper

The mechanism of Ca2+ stimulation of citrulline and N-acetylglutamate synthesis by mitochondria

Biochimica Et Biophysica Acta
J D Johnston, M D Brand

Abstract

ATP-driven citrulline synthesis by mitochondria treated with oligomycin, uncoupler and Ca2+ ionophore is stimulated more than 2-fold by an increase in extramitochondrial free [Ca2+] in the range 1-5 microM. Stimulation increases with the length of preincubation of mitochondria with Ca2+. EGTA prevents stimulation if added at the start of the preincubation period, but is without effect if added at the end, suggesting that Ca2+ acts indirectly on citrulline synthesis via the accumulation of an intermediate. Neither carbamoyl-phosphate synthase (ammonia) nor ornithine carbamoyltransferase are stimulated by up to 50 microM free Ca2+ in mitochondrial extracts, but N-acetylglutamate synthase is stimulated about 30% by 10 microM free Ca2+. We propose that an increase in the activity of N-acetylglutamate synthase in response to an increase in free [Ca2+] in the mitochondrial matrix may contribute to hormonal stimulation of the urea cycle.

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Citations

Dec 1, 1995·Journal of Bioenergetics and Biomembranes·S Soboll
Jan 1, 1991·The International Journal of Biochemistry·R Moreno-Sánchez, M E Torres-Márquez
Aug 27, 2005·Mitochondrion·Paul S Brookes
Nov 22, 2013·Advances in Virology·Sanjeev K Anand, Suresh K Tikoo
Nov 29, 2005·Neurotoxicology and Teratology·J MarxM S Bornman
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Paul S BrookesShey-Shing Sheu

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