PMID: 8963721May 1, 1996Paper

The mechanism of inhibition of factor III (thromboplastin) activity by apolipoprotein B-100. Protein-protein interactions

Arteriosclerosis, Thrombosis, and Vascular Biology
C EttelaieK R Bruckdorfer

Abstract

Factor III (thromboplastin) activity is inhibited by apoB-100, but the mechanism of inhibition is unknown. By examining the effect of purified apoB-100 on factor III activity, we showed that apoB-100 can inhibit factor III via a different mechanism from that caused by the issue-factor pathway-inhibitor, which is mainly carried on the surface of lipoproteins. Although the presence of calcium ions and factors X and VII may enhance the rate of inhibition, they are not a prerequisite for the inhibition of factor III by apoB-100. In addition, by investigating the changes in the UV spectra of apoB-100 on interaction with factor III and factors X and VII and by assigning the shifts in absorption spectra to particular amino acids, we showed that these interactions involve negative and positive residues within these proteins. By following the rates of interactions between apoB-100 and either factors III, X, VII, a two-step mechanism for the inhibition process involving factors X and VII was postulated. In this mechanism, the primary interaction of apoB-100 with factor III is followed by a rate-limiting step that can be accelerated by the presence of either factor X or VII and leads to the inhibition of factor III. Furthermore, a compute...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Aug 1, 1990·Biochemical Society Transactions·R M Howell, C Ettelaie
Jan 1, 1985·Annual Review of Cell Biology·J L GoldsteinW J Schneider
Jul 19, 1982·FEBS Letters·C Erlanson-Albertsson, H E Akerlund

❮ Previous
Next ❯

Citations

Jul 27, 2001·Archives of Biochemistry and Biophysics·A J ChuS Beydoun
Jun 29, 2001·British Journal of Pharmacology·A J ChuS Beydoun
Nov 13, 2001·British Journal of Haematology·A J ChuN Ramos
Jul 26, 2002·British Journal of Haematology·Arthur J ChuEvano Piasentin
Jun 2, 1998·Kidney International·P Carmeliet, D Collen
Mar 27, 2002·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·A J ChuS Beydoun
Jan 16, 1998·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K R Bruckdorfer

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.