The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells

International Journal of Molecular Sciences
Shujuan WangGuodong Liu

Abstract

Ovarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of MT2 in bovine GCs, and assess whether MT2 silencing affected GCs response to melatonin. We found that MT2 silencing significantly decreased the secretion of progesterone and estradiol, and increased the concentration of inhibin B and activin B. To further reveal the regulatory mechanism of MT2 silencing on steroids synthesis, it was found that the expression of CYP19A1 and CYP11A1 enzymes (steroid hormone synthesis) were down-regulated, while genes related to hormonal synthesis (StAR, RUNX2, INHA and INHBB) were up-regulated without affecting the expression of INHBA, suggesting that MT2 silencing may regulate hormone abundance. Furthermore, MT2 silencing significantly increased the expression of TGFBR3 and BMP6, and decreased the expression of LHR and DNMT1A without significant difference in the expression of FSHR and EGFR. In addition, MT2 silencing didn't affect the effect of melatonin on increasing the expression of DNMT1A, EGFR, INHBA and LHR, and progesterone level, or decreasing INHA, TGFBR3 and StAR expression, and production of inhibin B...Continue Reading

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Citations

Oct 22, 2019·Journal of Pineal Research·Nan FangLifeng Liu
Aug 14, 2020·Journal of Ovarian Research·Brandon A WyseClifford L Librach
Aug 14, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhuo-Tao LiangHong-Qi Zhang

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BETA
transfection
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