PMID: 8972697Dec 4, 1996Paper

The mechanism of monensin-mediated cation exchange based on real time measurements

Biochimica Et Biophysica Acta
Esther NachlielMenachem Gutman

Abstract

Monensin is an ionophore that supports an electroneutral ion exchange across the lipid bilayer. Because of this, under steady-state conditions, no electric signals accompany its reactions. Using the Laser Induced Proton Pulse as a synchronizing event we selectively acidify one face of a black lipid membrane impregnated by monensin. The short perturbation temporarily upsets the acid-base equilibrium on one face of the membrane, causing a transient cycle of ion exchange. Under such conditions the molecular events could be discerned as a transient electric polarization of the membrane lasting approx. 200 microseconds. The proton-driven chemical reactions that lead to the electric signals had been reconstructed by numeric integration of differential rate equations which constitute a maximalistic description of the multi equilibria nature of the system (Gutman, M. and Nachliel, E. (1989) Electrochim. Acta 34, 1801-1806). The analysis of the reactions reveals that the ionic selectivity of the monensin (H+ > Na+ > K+) is due to more than one term. Besides the well established different affinity for the various cations, the selectivity is also derived from a large difference in the rates of cross membranal diffusivities (MoH > MoNa > M...Continue Reading

References

Apr 29, 1992·Biochimica Et Biophysica Acta·B S Prabhananda, M H Kombrabail
Dec 4, 1996·Biochimica Et Biophysica Acta·S Bransburg-ZabaryM Gutman

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Citations

Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·C C Häse, J J Mekalanos
Jan 1, 1997·Annual Review of Physical Chemistry·M Gutman, E Nachliel
Oct 16, 2007·Drug and Chemical Toxicology·S N SanyalShailender Singh Kanwar
Jun 25, 2002·Biophysical Journal·Esther NachlielDieter Oesterhelt
Dec 4, 1996·Biochimica Et Biophysica Acta·S Bransburg-ZabaryM Gutman
May 23, 2002·Biophysical Journal·Jens Schamberger, Ronald J Clarke
Jan 14, 2003·Biophysical Journal·Bettina SchätzlerMenachem Gutman
Jun 23, 2010·The Journal of Cell Biology·Benjamin E SteinbergSergio Grinstein
Oct 27, 2017·FEMS Microbiology Letters·Alexander Mayer, Dirk Weuster-Botz
Aug 4, 2006·The Journal of Physical Chemistry. B·Adam HuczyñskiFranz Bartl

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