Abstract
Kinetics of inhibition of the two porcine pancreatic alpha-amylase components (PPA I and PPA II) by acarbose were performed using reduced DP18-maltodextrin and amylose as substrates. Similar Line-weaver-Burk primary plots were obtained. Two mixed non-competitive models are proposed. X-ray crystallographic data [Qian, M., Buisson, G., Duée. E., Haser, R. & Payan, F. (1994) Biochemistry 33, 6284-6294] are in support of the mixed non-competitive inhibition model which involves abortive complexes. Secondary plots are different; inhibition of reduced DP18-maltodextrin hydrolysis gives straight-lines plots while amylose gives parabolic curves. These results, confirmed by Dixon-plot analyses, allow us to postulate that, in inhibition of reduced DP18-maltodextrin hydrolysis, one molecule of acarbose is bound/ amylase molecule. In contrast, using amylose as a substrate, two molecules of acarbose are bound. These kinetically determined binding sites might correspond to surface sites found by X-ray crystallography [Qian, M., Haser, R. & Payan, F. (1995) Protein Sci. 4, 747-755]; the glucose site close to the active site and the maltose site, 2 nm away. In conclusion, no significant difference between PPA I and PPA II has been observed, ei...Continue Reading
References
Mar 1, 1978·European Journal of Biochemistry·P I Clark, G Lowe
Aug 1, 1978·Journal of Biochemistry·T SuganumaK Hiromi
Oct 1, 1977·Die Naturwissenschaften·D D SchmidtD Schäfer
Feb 1, 1975·FEBS Letters·M GrangerG Marchis-Mouren
Aug 21, 1992·Biochimica Et Biophysica Acta·K Takase
Nov 15, 1991·Biochimica Et Biophysica Acta·V DesseauxG Marchis-Mouren
Jun 1, 1990·Trends in Biochemical Sciences·G K Farber, G A Petsko
Jul 3, 1990·Biochemistry·E BoelH F Woldike
Apr 1, 1985·European Journal of Biochemistry·C SeignerG Marchis-Mouren
Jan 21, 1972·European Journal of Biochemistry·P ElödiM Krysteva
Dec 1, 1965·Analytical Biochemistry·S DygertJ A Thoma
Jun 27, 1967·Biochimica Et Biophysica Acta·G Marcjis-Mouren, L Paséro
Apr 10, 1984·Biochemistry·E R Wilcox, J R Whitaker
Mar 1, 1984·Journal of Biochemistry·Y MatsuuraM Kakudo
Apr 1, 1995·Protein Science : a Publication of the Protein Society·M QianF Payan
Mar 17, 1995·Carbohydrate Research·E H Ajandouz, G J Marchis-Mouren
May 24, 1994·Biochemistry·M QianF Payan
May 27, 1994·Journal of Molecular Biology·A KadziolaR Haser
Nov 1, 1993·European Journal of Biochemistry·C SchouM Schülein
Jun 5, 1993·Journal of Molecular Biology·M QianF Payan
Sep 1, 1995·Protein Science : a Publication of the Protein Society·G D BrayerS G Withers
Dec 1, 1995·Protein Science : a Publication of the Protein Society·Y Liu, J M Sturtevant
Jun 1, 1996·European Journal of Biochemistry·C GillesF Payan
Feb 12, 1963·Biochimica Et Biophysica Acta·W W CLELAND
Citations
Feb 12, 1998·Protein Science : a Publication of the Protein Society·M QianF Payan
Feb 12, 2004·Biochimica Et Biophysica Acta·Birte SvenssonBirgit C Bønsager
Feb 12, 2004·Biochimica Et Biophysica Acta·Marius SantimoneVéronique Desseaux
Jul 10, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shigeru HokariTsugikazu Komoda
Jan 1, 1997·Progress in Biophysics and Molecular Biology·S Janecek
Aug 1, 1997·International Journal of Biological Macromolecules·M AlkazazM Santimone
Oct 17, 1998·Biochimica Et Biophysica Acta·G Ferey-RouxM Santimone
Mar 20, 1999·Biochimica Et Biophysica Acta·S DarnisJ C Chaix
Mar 16, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Y MoreauM Santimone
Nov 15, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P TalamondG Marchis-Mouren
Jun 5, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shigeru HokariTsugikazu Komoda
Dec 21, 2002·European Journal of Biochemistry·Hans LeemhuisLubbert Dijkhuizen
Sep 27, 2003·European Journal of Biochemistry·Naïma OudjeriouatVéronique Desseaux
Sep 22, 1999·European Journal of Biochemistry·R KoukiekoloM Santimone
Jul 3, 1998·The EMBO Journal·M LauwereysS Muyldermans
Oct 15, 2013·Pharmacognosy Magazine·Jie TuYoubing Liu
Feb 5, 2013·FEBS Letters·Elaheh Kashani-AminAzadeh Ebrahim-Habibi
Dec 17, 2014·Carbohydrate Polymers·Frederick J WarrenSushil Dhital
Jul 27, 2014·Transgenic Research·Y S LinW T K Cheng
Apr 21, 2016·Journal of Agricultural and Food Chemistry·Caroline Mary RyanAndrew Peter Neilson
Mar 27, 2004·FEMS Microbiology Letters·Hee Kyoung KangTae-Wha Moon
Feb 17, 2001·Rapid Communications in Mass Spectrometry : RCM·D J DouglasV J Nesatyy
Jul 4, 2012·Chembiochem : a European Journal of Chemical Biology·Mario Roberto SengerFloriano Paes Silva
Feb 13, 2001·European Journal of Biochemistry·R KoukiekoloM Santimone
Jun 8, 2017·Enzyme Research·Camila Gabriel KatoRosane Marina Peralta
May 31, 2008·The Journal of Biological Chemistry·Marc Saura-VallsAntoni Planas
Nov 23, 2020·Enzyme and Microbial Technology·Masako OgasawaraKuniyo Inouye
Nov 5, 1999·Archives of Biochemistry and Biophysics·M J KimK H Park
Jun 20, 1998·Journal of Molecular Biology·S StroblR Glockshuber
Jan 15, 1998·Biochimica Et Biophysica Acta·V Le Berre-AntonP Rougé
Apr 4, 2001·Carbohydrate Research·S B LeeJ F Robyt
Mar 24, 2010·Journal of Agricultural and Food Chemistry·Yadahally N SreeramaVishwas M Pratape
Mar 15, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pascale TalamondAlexandre de Kochko
Jul 18, 2002·Biochemistry·Myo-Jeong KimKwan-Hwa Park