PMID: 9523717Apr 2, 1998Paper

The mechanism of porcine pancreatic alpha-amylase. Inhibition of maltopentaose hydrolysis by acarbose, maltose and maltotriose

European Journal of Biochemistry
M Al KazazM Santimone

Abstract

A kinetic study was carried out on the inhibitory effects of acarbose, maltose, and maltotriose on porcine pancreatic alpha-amylase (PPA), using maltopentaose as the substrate. Lineweaver-Burk plots showed that the inhibitory action of acarbose is of the mixed non-competitive type. The secondary plots gave straight lines. A model involving abortive complexes accounts for these results. Dixon plot analysis led to the same conclusion. According to the proposed model, one molecule of acarbose per amylase molecule binds either directly to free enzyme at the active site or to the enzyme-substrate complex at a secondary carbohydrate-binding site, which becomes functional after the substrate has bound to the enzyme molecule at the active site. Kinetic analysis of the inhibition exerted by either the maltose or maltotriose reaction products of maltopentaose hydrolysis were then performed. The inhibitory effect of maltose was found to be of the non-competitive type, while that of maltotriose was competitive. It can therefore be concluded that the first reaction product to be released upon maltopentaose hydrolysis is maltose, and that the second product is maltotriose. This indicates that after hydrolysis of the maltopentaose chain, the ...Continue Reading

Citations

Jun 27, 2012·Bioprocess and Biosystems Engineering·Ana Vrsalović PresečkiÐurđa Vasić-Rački
Jan 16, 2003·Journal of Molecular Biology·Narayanan RamasubbuPrasunkumar J Mishra
Aug 13, 2011·Journal of Agricultural and Food Chemistry·Tingting LiuDejian Huang
Dec 21, 2002·European Journal of Biochemistry·Hans LeemhuisLubbert Dijkhuizen
Sep 27, 2003·European Journal of Biochemistry·Naïma OudjeriouatVéronique Desseaux
Jan 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Buford L NicholsErwin Sterchi
Nov 26, 2010·Plant Physiology·Duncan StanleyAlison M Smith
Feb 24, 2001·Bioscience, Biotechnology, and Biochemistry·J S KimK H Son
Dec 11, 2013·Applied Microbiology and Biotechnology·Flora TsvetanovaKaloyan Petrov
Jul 8, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Fernando PorcelliGianluigi Veglia
Nov 26, 2003·Biochemical and Biophysical Research Communications·Gyöngyi GyémántLászló Somsák
Dec 17, 2005·Chemistry & Biology·Lucie Dolecková-MaresováMichael Mares
May 30, 2008·Journal of Medicinal Chemistry·Elena Lo PiparoChieh Jason Chou

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