PMID: 6172343Jan 1, 1981Paper

The mechanism of resistance to streptomycin in Escherichia coli. Functional analysis of the permeability barrier of cells harbouring the R1 drd-19Km- plasmid

Folia Microbiologica
H BranáI Holubová

Abstract

The mechanism of phenotypically altered SM resistance in mutants of Escherichia coli JC5455 (Rldrd-19Km-) lrs and JC5455 (pON5300) was compared with that of the standard strain JC5455 (Rldrd-19Km-). On analyzing the membrane polypeptides in polyacrylamide gel both mutants were found to possess a protein spectrum different from that of ths standard strain. Transport of D-xylose and L-arginine was the same in all strains, transport of L-proline was decreased in JC5455 (pON5300) which may indicate a mutational interference with energy metabolism. The basic uptake of dihydrostreptomycin was the same in all strains but there were differences after preincubation of cells with streptomycin or glucose. The increased resistance of JC5455 (Rldrd-19Km-) lrs may be due to observed quantitative differences in membrane polypeptides that might play a role in the binding and functional expression of aminoglycoside-3'adenylyl transferase which modifies streptomycin. The increased sensitivity toward streptomycin in JC5455 (pON5300) can be explained by a mutation due to N-methyl-N'-nitro-N-nitrosoguanidine in the host cell since this change of sensitivity to streptomycin could not be transferred by transformation into a nonmutagenized strain. The...Continue Reading

References

Jan 1, 1978·Annual Review of Microbiology·J Davies, D I Smith
Feb 1, 1979·Antimicrobial Agents and Chemotherapy·J V Höltje
Jun 1, 1976·Antimicrobial Agents and Chemotherapy·L E Bryan, H M Van den Elzen
Apr 2, 1975·Biochimica Et Biophysica Acta·G O HumphreysE S Anderson
Jan 1, 1973·Folia Microbiologica·H BranáJ König
Nov 1, 1973·Journal of Bacteriology·P GuerryS Falkow

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Citations

Nov 19, 2010·Journal of Industrial Microbiology & Biotechnology·Jan Nešvera

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