May 25, 1976

The mechanism of superoxide anion generation by the interaction of phenylhydrazine with hemoglobin

The Journal of Biological Chemistry
B GoldbergJ Peisach

Abstract

The mechanism by which superoxide anion is generated by the interaction of phenylhydrazine with either oxy- or methemoglobin was investigated. Rather than superoxide anion generation resulting from an accelerated autooxidation of oxyhemoglobin, it was found that both oxy- and methemoglobin function as peroxidases toward phenylhydrazine with the resultant oxidation of this compound to phenyldiazine. Generation of phenyldiazine from the oxidation of phenylhydrazine by hemoglobin or by the hydrolysis and subsequent decarboxylation of methyl phenylazoformate (C6H5N=NCOOCH3) resulted in the production of superoxide anion. It is suggested that under certain conditions hemoglobin may function as a drug-metabolizing peroxidase.

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Mentioned in this Paper

Methemoglobin
Dioxygen
Peroxidases
Benzene
Superoxides
Oxyhemoglobin Measurement
Oxidation
Methemoglobin Measurement
Azoester
Medihaler-Epi

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